MATERNAL CREATINE SUPPLEMENTATION AFFECTS THE MORPHO-FUNCTIONAL DEVELOPMENT OF HIPPOCAMPAL NEURONS IN RAT OFFSPRING
MATERNAL CREATINE SUPPLEMENTATION AFFECTS THE MORPHO-FUNCTIONAL DEVELOPMENT OF HIPPOCAMPAL NEURONS IN RAT OFFSPRING
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DOI：
10.1016/j.neuroscience.2015.11.017
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发表时间：
2016-01-15
期刊：
影响因子：
3.3
通讯作者：
Cuppini, R.
中科院分区：
文献类型：
--
作者：
Sartini, S.;Lattanzi, D.;Cuppini, R.
肌酸补充已被证明可以保护神经元免受氧化损伤，这是因为它具有抗氧化和麦角化功能。这些特点导致了在怀孕期间补充肌酸作为预防中枢神经系统损伤的预防性治疗的假说，如缺氧缺血性脑病。不幸的是，对神经元分化过程中补充肌酸的影响知之甚少，而体外研究显示了对神经元兴奋性的影响，这使得在中枢神经系统发育期间补充肌酸的可能性成为一个悬而未决的问题。本文采用多种方法研究了孕期饮水中添加1%肌酸对新生大鼠海马神经元形态和功能发育的影响。与对照组相比，添加补充剂的新生大鼠CA1区锥体神经元表现出树突发育增强，长时程增强，诱发突触反应增加，内在兴奋性增强。此外，肌酸处理组神经元记录到更快的动作电位复极期，并出现超极化现象。一直以来，肌酸暴露组小鼠的CA1神经元表现出比对照组更高的最大放电频率。综上所述，我们发现孕期补充肌酸对子代大鼠CA1神经元的形态和电生理发育有积极的影响，增加了神经元的兴奋性。总之，这些发现强调了评估母体摄取肌酸对人类的益处和安全性的必要性。(C)2015年IBRO。爱思唯尔有限公司出版。保留所有权利。
Creatine supplementation has been shown to protect neurons from oxidative damage due to its antioxidant and ergogenic functions. These features have led to the hypothesis of creatine supplementation use during pregnancy as prophylactic treatment to prevent CNS damage, such as hypoxic-ischemic encephalopathy. Unfortunately, very little is known on the effects of creatine supplementation during neuron differentiation, while in vitro studies revealed an influence on neuron excitability, leaving the possibility of creatine supplementation during the CNS development an open question. Using a multiple approach, we studied the hippocampal neuron morphological and functional development in neonatal rats born by dams supplemented with 1% creatine in drinking water during pregnancy. CA1 pyramidal neurons of supplemented newborn rats showed enhanced dendritic tree development, increased LTP maintenance, larger evoked-synaptic responses, and higher intrinsic excitability in comparison to controls. Moreover, a faster repolarizing phase of action potential with the appearance of a hyperpolarization were recorded in neurons of the creatine-treated group. Consistently, CA1 neurons of creatine exposed pups exhibited a higher maximum firing frequency than controls. In summary, we found that creatine supplementation during pregnancy positively affects morphological and electrophysiological development of CA1 neurons in offspring rats, increasing neuronal excitability. Altogether, these findings emphasize the need to evaluate the benefits and the safety of maternal intake of creatine in humans. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.