Analysis of the serum antibody responses to type 1 and type 2 fimbriae in mice immunized with Actinomyces viscosus T14V.
Analysis of the serum antibody responses to type 1 and type 2 fimbriae in mice immunized with Actinomyces viscosus T14V.
复制标题
粘性放线菌 T14V 免疫小鼠对 1 型和 2 型菌毛的血清抗体反应分析。
DOI：
10.1111/j.1600-0765.1989.tb00861.x
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发表时间：
1989
影响因子：
3.5
通讯作者：
Grinnell,C
中科院分区：
文献类型：
--
作者：
Haber,J;Grinnell,C
采用酶联免疫吸附法（elisa）测定与a反应的血清抗体，分析了用放线菌粘索14v细胞免疫近交系小鼠的抗体反应。vissusl4v细胞和1型和2型被膜。A/I小鼠在第0天和第14天腹腔免疫，血清抗体反应在第19-35天达到峰值，108个细胞/次注射的剂量达到最佳反应。对三种基因不同的近交系小鼠的反应分析表明，抗1型抗体（6.5倍）和抗2型抗体（14.3倍）的数量存在显著差异。观察到的抗虫膜抗体反应强度的变化在理论上足以解释不同小鼠品系在抑制a.l usus14v粘附唾液包覆的羟基磷灰石和其他细菌能力上的显著差异。这些研究提供了一个模型，可以分析抗毛膜抗体反应的变化对细菌粘附的影响。
The antibody responses of inbred mice immunized withActinomyces viscosusT14V cells were analyzed using enzyme‐linked immunoabsorbent assays (ELISAs) for measuring serum antibodies reactive withA. viscosusTl4V cells and type 1 and type 2 fImbriae. In A/I mice immunized intraperitoneally on days 0 and 14, the serum antibody responses approached peak levels during d 19–35, and a dose of 108cells/injection elicited optimal responses. Analysis of the responses of three genetically diverse strains of inbred mice indicated striking variations in the amounts of anti‐type 1 (6.5‐fold) and anti‐type 2 (14.3‐fold) antibodies elicited. The observed variations in the magnitude of the anti‐fImbrial antibody responses are theoretically of sufficient magnitude to account for signifIcant differences between mouse strains in their ability to inhibit adherence of A.viscosusT14V to saliva‐coated hydroxyapatite and other bacteria. These studies provide a model with which the effects of variations in anti‐fimbrial antibody responses on bacterial adherence may be analyzed.