The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants.
The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants.
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DOI：
10.3389/fpls.2017.00392
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发表时间：
2017
影响因子：
5.6
通讯作者：
Ruyter-Spira C
中科院分区：
文献类型：
--
作者：
Cheng X;Floková K;Bouwmeester H;Ruyter-Spira C
根寄生植物物种多枝兰（英语：Bleedicipanche ramosa），即分枝列当（英语：branched broomrape），对番茄等重要经济作物造成严重损害。它的种子萌发是由宿主来源的信号触发的，它侵入宿主的根。在番茄中，独脚金内酯（SL）是多枝番茄的主要发芽刺激物。因此，低SL生产线的发展可能是一种方法来解决寄生杂草的问题。然而，由于SL也是控制植物发育的许多方面的植物激素，SL缺乏也可能对感染过程的萌发后阶段产生影响，在寄生虫-宿主相互作用期间。在这项研究中，我们表明，SL-缺陷番茄植物（番茄; SLCCD 8 RNAi线），感染预发芽的P. ramosa种子，显示感染水平的增加和更快的发展的寄生虫，这表明SL在宿主防御寄生植物入侵的积极作用。此外，我们表明，SL-缺陷型番茄植株失去了他们的特征SL-缺陷型感染期间，通过减少节间的数量和二次分枝的数量和长度与P. ramosa。多枝假单胞菌感染导致野生型和SL缺陷株系的叶和根中脱落酸（阿坝）水平增加。寄生虫感染后，ABA-葡萄糖酯（ABA-GE）的共轭水平也增加了野生型和SL-缺陷线的叶和一个SL-缺陷线的根。未感染的SL-缺陷系具有比野生型更高的叶片ABA-GE水平。尽管阿坝水平高，气孔开度和水分损失率不受SL-缺陷线寄生虫感染，而在野生型番茄气孔开度和水分损失增加感染后。未来的研究需要进一步巩固SL在寄主与寄生植物的相互作用中发挥的作用，以及在此过程中其他植物激素与SL的相互作用。
The root parasitic plant species Phelipanche ramosa, branched broomrape, causes severe damage to economically important crops such as tomato. Its seed germination is triggered by host-derived signals upon which it invades the host root. In tomato, strigolactones (SLs) are the main germination stimulants for P. ramosa. Therefore, the development of low SL-producing lines may be an approach to combat the parasitic weed problem. However, since SLs are also a plant hormone controlling many aspects of plant development, SL deficiency may also have an effect on post-germination stages of the infection process, during the parasite-host interaction. In this study, we show that SL-deficient tomato plants (Solanum lycopersicum; SlCCD8 RNAi lines), infected with pre-germinated P. ramosa seeds, display an increased infection level and faster development of the parasite, which suggests a positive role for SLs in the host defense against parasitic plant invasion. Furthermore, we show that SL-deficient tomato plants lose their characteristic SL-deficient phenotype during an infection with P. ramosa through a reduction in the number of internodes and the number and length of secondary branches. Infection with P. ramosa resulted in increased levels of abscisic acid (ABA) in the leaves and roots of both wild type and SL-deficient lines. Upon parasite infection, the level of the conjugate ABA-glucose ester (ABA-GE) also increased in leaves of both wild type and SL-deficient lines and in roots of one SL-deficient line. The uninfected SL-deficient lines had a higher leaf ABA-GE level than the wild type. Despite the high levels of ABA, stomatal aperture and water loss rate were not affected by parasite infection in the SL-deficient line, while in wild type tomato stomatal aperture and water loss increased upon infection. Future studies are needed to further underpin the role that SLs play in the interaction of hosts with parasitic plants and which other plant hormones interact with the SLs during this process.