Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea.
Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea.
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DOI：
10.1038/nbt.3893
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发表时间：
2017-08-08
影响因子：
46.9
通讯作者：
Woyke T
中科院分区：
文献类型：
--
作者：
Bowers RM;Kyrpides NC;Stepanauskas R;Harmon-Smith M;Doud D;Reddy TBK;Schulz F;Jarett J;Rivers AR;Eloe-Fadrosh EA;Tringe SG;Ivanova NN;Copeland A;Clum A;Becraft ED;Malmstrom RR;Birren B;Podar M;Bork P;Weinstock GM;Garrity GM;Dodsworth JA;Yooseph S;Sutton G;Glöckner FO;Gilbert JA;Nelson WC;Hallam SJ;Jungbluth SP;Ettema TJG;Tighe S;Konstantinidis KT;Liu WT;Baker BJ;Rattei T;Eisen JA;Hedlund B;McMahon KD;Fierer N;Knight R;Finn R;Cochrane G;Karsch-Mizrachi I;Tyson GW;Rinke C;Genome Standards Consortium;Lapidus A;Meyer F;Yilmaz P;Parks DH;Eren AM;Schriml L;Banfield JF;Hugenholtz P;Woyke T
提出了对微生物“未培养的多数”，即细菌和古细菌单细胞基因组序列和来自宏基因组数据集的基因组序列进行测序的标准。本文的在线版本（doi：10.1038/nbt.3893）包含补充材料，可供授权用户使用。我们提出了两个标准的基因组标准协会（GSC）报告细菌和古细菌基因组序列。它们都是关于任意（x）序列的最小信息（MIxS）的扩展。这些标准是关于单个扩增基因组的最小信息（MISAG）和关于宏基因组组装基因组的最小信息（MIMAG），包括但不限于组装质量以及基因组完整性和污染的估计。这些标准可以与其他GSC清单结合使用，包括关于基因组序列的最小信息（MIGS），关于宏基因组序列的最小信息（MIMS）和关于标记基因序列的最小信息（MIMARKS）。在社区范围内采用MISAG和MIMAG将有助于对细菌和古菌多样性进行更有力的比较基因组分析。本文的在线版本（doi：10.1038/nbt.3893）包含补充材料，可供授权用户使用。
Standards for sequencing the microbial 'uncultivated majority', namely bacterial and archaeal single-cell genome sequences, and genome sequences from metagenomic datasets, are proposed. The online version of this article (doi:10.1038/nbt.3893) contains supplementary material, which is available to authorized users. We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity. The online version of this article (doi:10.1038/nbt.3893) contains supplementary material, which is available to authorized users.
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影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
64.8
作者：
Brown, Christopher T.;Hug, Laura A.;Banfield, Jillian F.
通讯作者：
Banfield, Jillian F.
影响因子：
2.7
作者：
Darling AE;Jospin G;Lowe E;Matsen FA 4th;Bik HM;Eisen JA
通讯作者：
Eisen JA
DOI：
10.1126/science.1180614
发表时间：
2009-10-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chain PS;Grafham DV;Fulton RS;Fitzgerald MG;Hostetler J;Muzny D;Ali J;Birren B;Bruce DC;Buhay C;Cole JR;Ding Y;Dugan S;Field D;Garrity GM;Gibbs R;Graves T;Han CS;Harrison SH;Highlander S;Hugenholtz P;Khouri HM;Kodira CD;Kolker E;Kyrpides NC;Lang D;Lapidus A;Malfatti SA;Markowitz V;Metha T;Nelson KE;Parkhill J;Pitluck S;Qin X;Read TD;Schmutz J;Sozhamannan S;Sterk P;Strausberg RL;Sutton G;Thomson NR;Tiedje JM;Weinstock G;Wollam A;Genomic Standards Consortium Human Microbiome Project Jumpstart Consortium;Detter JC
通讯作者：
Detter JC
影响因子：
5.4
作者：
Gilbert JA;Jansson JK;Knight R
通讯作者：
Knight R