Neonatal Salivary Analysis Reveals Global Developmental Gene Expression Changes in the Premature Infant
Neonatal Salivary Analysis Reveals Global Developmental Gene Expression Changes in the Premature Infant
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DOI：
10.1373/clinchem.2009.136234
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发表时间：
2010-03-01
影响因子：
9.3
通讯作者：
Bianchi, Diana W.
中科院分区：
文献类型：
--
作者：
Maron, Jill L.;Johnson, Kirby L.;Bianchi, Diana W.
背景：有一个重要的需求，开发非侵入性的生物标志物来检测早产儿疾病。我们的目的是确定唾液基因组分析是否提供了新的信息，新生儿的发育genes.METHODS表达：唾液（50-200 μ L）前瞻性收集5个早产儿在5个时间点：前，开始，并推进肠内营养;在引入口服饲料;并在先进的口服饲料。唾液RNA提取，扩增，并杂交到全基因组microarrais.Results：生物信息学分析确定了9286个基因转录与统计学显着的基因表达变化，随着时间的推移，在个人。在这些基因中，3522个（37.9%）下调，5764个（62.1%）上调。基因表达变化与发育途径高度相关。在胚胎发育、结缔组织发育和功能、血液系统发育和功能以及生物体存活中观察到显著下调的表达（10（-14）< p < 10（-3））。相反，与行为、神经系统发育、组织发育、器官发育和消化系统发育相关的基因显著上调（10（-11）< P < 10（-2））。结论：比较基因组唾液分析提供了关于发育中早产儿几乎所有器官和组织的可靠、全面、实时的信息。这种创新和非侵入性的技术代表了一种新的方法来监测健康，疾病和发展，在这个脆弱的患者群体。通过将健康婴儿的这些数据与发生医学并发症的婴儿的数据进行比较，我们希望能够确定新的生物标志物，最终改善新生儿护理。(C)2009年美国临床化学协会
BACKGROUND: There is an important need to develop noninvasive biomarkers to detect disease in premature neonates. Our objective was to determine if salivary genomic analysis provides novel information about neonatal expression of developmental genes.METHODS: Saliva (50-200 mu L) was prospectively collected from 5 premature infants at 5 time points: before, starting, and advancing enteral nutrition; at the introduction of oral feeds; and at advanced oral feeds. Salivary RNA was extracted, amplified, and hybridized onto whole-genomic microarrays.RESULTS: Bioinformatics analyses identified 9286 gene transcripts with statistically significant gene expression changes across individuals over time. Of these genes, 3522 (37.9%) were downregulated, and 5764 (62.1%) were upregulated. Gene expression changes were highly associated with developmental pathways. Significantly downregulated expression was seen in embryonic development, connective tissue development and function, hematologic system development and function, and survival of the organism (10(-14) < p < 10(-3)). Conversely, genes associated with behavior, nervous system development, tissue development, organ development, and digestive system development were significantly upregulated (10(-11) < P < 10(-2)).CONCLUSIONS: Comparative genomic salivary analyses provide robust, comprehensive, real-time information regarding nearly all organs and tissues in the developing preterm infant. This innovative and noninvasive technique represents a new approach for monitoring health, disease, and development in this vulnerable patient population. By comparing these data in healthy infants with data from infants who develop medical complications, we expect to identify new biomarkers that will ultimately improve newborn care. (C) 2009 American Association for Clinical Chemistry