Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy.
Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy.
复制标题
用于成像宽视野显微镜中的傅立叶转换光谱的接近路径干涉仪。
DOI：
10.1364/optica.4.000546
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发表时间：
2017-05-20
期刊：
影响因子：
10.4
通讯作者：
So PTC
中科院分区：
文献类型：
--
作者：
Wadduwage DN;Singh VR;Choi H;Yaqoob Z;Heemskerk H;Matsudaira P;So PTC
成像傅里叶变换光谱(IFTS)是一种基于荧光或拉曼等多种成像方式进行生物高光谱分析的有效方法。由于测量是在频谱的傅里叶空间中进行的，因此它还可以利用压缩感知策略。IFTS已经很容易地在基于广域成像模式的高通量、高含量的显微镜系统中实现。然而，现有的广域IFTS设计存在局限性。非公共路径方法的相位稳定性较差。或者，基于公共路径Sagnac干涉仪的设计是稳定的，但与高通量成像不兼容。它们需要对大的干涉路径延迟进行详尽的顺序扫描，这使得压缩战略数据获取变得不可能。在本文中，我们提出了一种基于战略数据采集的新型相位稳定、近公共路径干涉仪，能够实现高通量高光谱成像。我们的结果表明，这种方法可以在不影响相位稳定性的情况下，将吞吐量提高超过许多其他广域光谱技术的一个数量级。
Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strategies. IFTS has been readily implemented in high-throughput, high-content microscope systems based on wide-field imaging modalities. However, there are limitations in existing wide-field IFTS designs. Non-common-path approaches are less phase-stable. Alternatively, designs based on the common-path Sagnac interferometer are stable, but incompatible with high-throughput imaging. They require exhaustive sequential scanning over large interferometric path delays, making compressive strategic data acquisition impossible. In this paper, we present a novel phase-stable, near-common-path interferometer enabling high-throughput hyperspectral imaging based on strategic data acquisition. Our results suggest that this approach can improve throughput over those of many other wide-field spectral techniques by more than an order of magnitude without compromising phase stability.