Partial transformation of human thyroid epithelial cells by mutant Ha-ras oncogene.
Partial transformation of human thyroid epithelial cells by mutant Ha-ras oncogene.
复制标题
突变型 Ha-ras 癌基因对人甲状腺上皮细胞的部分转化。
作者：
N. Lemoine;S. Staddon;J. Bond;F. Wyllie;J. Shaw;D. Wynford‐Thomas
我们先前已经证明ras癌基因突变激活是甲状腺肿瘤的常见早期事件。使用嗜酸性逆转录病毒载体，以实现基因转移，我们在这里证明，人原发性甲状腺上皮细胞可以部分转化的激活细胞或病毒的Ha-ras癌基因，在没有一个合作的癌基因。由ras诱导的转化事件涉及从衰老中暂时拯救多达20轮的细胞分裂，以及形态学改变、生长因子独立性和锚定独立性。因此，有可能在体外重建人类上皮瘤形成的关键早期事件。
We have previously shown that activation of ras oncogenes by mutation is a frequent early event in human thyroid neoplasia. Using amphotropic retroviral vectors to achieve gene transfer, we demonstrate here that human primary thyroid epithelial cells can be partially transformed by an activated cellular or viral Ha-ras oncogene, in the absence of a cooperating oncogene. The transformation event induced by ras involves temporary rescue from senescence for up to 20 rounds of cell division together with morphological alteration, growth factor independence and anchorage independence. It has therefore been possible to reconstruct in vitro a key early event in the genesis of human epithelial neoplasia.