New approach of monitoring changes in chlorophyll a fluorescence of single guard cells and protoplasts in response to physiological stimuli
New approach of monitoring changes in chlorophyll a fluorescence of single guard cells and protoplasts in response to physiological stimuli
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监测叶绿素变化的新方法以及单个保卫细胞和原生质体响应生理刺激的荧光变化
DOI：
10.1046/j.1365-3040.1999.00475.x
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发表时间：
1999
影响因子：
7.3
通讯作者：
R. Hedrich
中科院分区：
文献类型：
--
作者：
C. Goh;U. Schreiber;R. Hedrich
应用一种新型的微型荧光仪，利用饱和脉冲法，通过叶绿素荧光在单细胞水平上评价光合作用。将显微脉冲调幅(PAM)叶绿素荧光仪与Zeiss Axiovert 25倒置荧光显微镜相结合，对单个叶肉细胞、保卫细胞以及相应的原生质体进行了高分辨率测量。可利用的信息包括光系统II的有效量子产率、相对电子传输速率和由于跨类囊体质子梯度而使类囊体膜的能量增加。保卫细胞(GCPs)和叶肉细胞原生质体(MCPs)的暗光诱导曲线表现出非常相似的特征，表明两种类型叶绿体中类囊体膜的功能结构相似。然而，光响应曲线显示，GCPs的光合作用电子流比MCPs早得多。在厌氧条件下，光合作用电子流和细胞膜能化受到严重抑制。当表皮细胞与真菌毒素褐变素孵育时，在保卫细胞中也观察到类似的效应，该毒素激活质膜H+-ATPase，导致气孔不可逆的开放。电子传递速率的下降可通过阻断H+泵的ATP消耗或添加葡萄糖来防止。这些结果表明，叶绿素荧光猝灭分析可以对气孔生理有更深刻的了解。
A new type of microfluorometer was applied to assess photosynthesis at the single-cell level by chlorophyll fluorescence using the saturation pulse method. A microscopy-pulse amplitude modulation (PAM) chlorophyll fluorometer was combined with a Zeiss Axiovert 25 inverted epifluorescence microscope for high-resolution measurements on single mesophyll and guard cells and the respective protoplasts. Available information includes effective quantum yield of photosystem II, relative electron transport rate and energization of the thylakoid membrane due to the transthylakoidal proton gradient. Dark-light induction curves of guard cell (GCPs) and mesophyll cell protoplasts (MCPs) displayed very similar characteristics, indicating similar functional organization of thylakoid membranes in both types of chloroplasts. Light response curves, however, revealed much earlier saturation of photosynthetic electron flow in GCPs than in MCPs. Under anaerobiosis, photosynthetic electron flow and membrane energization were severely suppressed. A similar effect was observed in guard cells when epidermal peels were incubated with the fungal toxin fusicoccin which activates the plasma membrane H + -ATPase and causes irreversible opening of stomata. The drop in electron transport rate was prevented by blocking ATP consumption of the H + pump or by glucose addition. These results show that chlorophyll fluorescence quenching analysis allows profound insights into stomatal physiology.
保卫细胞叶绿体和叶肉细胞叶绿体之间的蛋白质组比较
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Boseok Song;Sho Yamagaki;Sakura Nishimura;Jo Narimatsu;Koh Iba;Juntaro Negi
通讯作者：
Juntaro Negi