Comparative transcriptome analysis of NaCl and KCl stress response in Malus hupehensis Rehd. Provide insight into the regulation involved in Na+ and K+ homeostasis
Comparative transcriptome analysis of NaCl and KCl stress response in Malus hupehensis Rehd. Provide insight into the regulation involved in Na+ and K+ homeostasis
复制标题
湖北海棠 NaCl 和 KCl 胁迫反应的比较转录组分析。
DOI：
10.1016/j.plaphy.2021.04.022
复制
发表时间：
2021-05-08
影响因子：
6.5
通讯作者：
Wang, Caihong
中科院分区：
文献类型：
--
作者：
Li, Yuqi;Zheng, Xiaodong;Wang, Caihong
背景：苹果是世界上种植最广泛的多年生水果作物之一。苹果对盐胁迫非常敏感，盐胁迫会破坏苹果体内Na+、K+的平衡，严重影响苹果树的生长和生产力。以往的研究主要集中在NaCl胁迫下的分子机制。结果：综合分析了湖北海棠的耐盐性，其是苹果园中广泛使用的砧木。根和叶用NaCl和KCl处理。通过作图分析，共鉴定出762个与NaCl和KCl胁迫相关的根和叶差异表达基因（DEG）。此外，我们通过WGCNA分析鉴定了七个枢纽基因。基因本体论（GO）的条款丰富的离子跨膜转运蛋白和氧化还原酶活性在NaCl和KCl胁迫下。京都基因与基因组百科全书（KEGG）主要研究植物激素信号转导和丝裂原活化蛋白激酶信号转导途径。我们还从762个DEG中筛选出28个候选基因，并通过定量逆转录聚合酶链反应（qRT-PCR）验证其表达。这些富集基因与NaCl和KCl胁迫密切相关，参与调节M. hupehensis.Conclusions:该转录组分析为阐明NaCl和KCl胁迫的信号转导途径提供了宝贵的资源，并且是发现NaCl和KCl胁迫响应相关基因的重要遗传资源。
Background: Apple is among the most widely cultivated perennial fruit crops worldwide. It is sensitive to salt stress, which seriously affects the growth and productivity of apple trees by destroying the homeostasis of Na+ and K+. Previous studies focused on the molecular mechanism underlying NaCl stress. However, signaling transduction under KCl stress has not been thoroughly studied.Results: We comprehensively analyzed the salt tolerance of Malus hupehensis Rehd., which is a widely used rootstock in apple orchards, by using RNA-Seq. Roots and leaves were treated with NaCl and KCl. Based on mapping analyses, a total of 762 differentially expressed genes (DEGs) related to NaCl and KCl stress in the roots and leaves were identified. Furthermore, we identified seven hub genes by WGCNA Analysis. The Gene Ontology (GO) terms were enriched in ion transmembrane transporter and oxidoreductase activity under NaCl and KCl stress. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways focused on the plant hormone signal transduction and mitogen-activated protein kinase signaling pathway. We also screened out 28 candidate genes from 762 DEGs and verified their expression by quantitative reverse transcription polymerase chain reaction (qRT-PCR). All of these enriched genes were closely related to NaCl and KCl stress and take part in mediating the Na+ and K+ homeostasis in M. hupehensis.Conclusions: This transcriptome analysis provides a valuable resource for elucidating the signaling pathway of NaCl and KCl stress and is a substantial genetic resource for discovering genes related to the NaCl and KCl stress response.