Effect of membrane tension on the electric field and dipole potential of lipid bilayer membrane.
Effect of membrane tension on the electric field and dipole potential of lipid bilayer membrane.
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DOI：
10.1016/j.bbamem.2011.06.010
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发表时间：
2011-10
影响因子：
3.4
通讯作者：
Chachisvilis, Mirianas
中科院分区：
文献类型：
--
作者：
Warshaviak, Dora Toledo;Muellner, Michael J.;Chachisvilis, Mirianas
脂质双层膜的偶极电位控制膜对带相反电荷的离子的渗透性差异。我们结合了分子动力学 (MD) 模拟和实验研究，以确定 1,2-二油酰-sn-甘油-3-磷酸胆碱 (DOPC) 脂质双层响应施加的膜张力的电场和静电势的变化。基于 CHARMM36 力场的 MD 模拟表明，DOPC 双层的静电势在膜张力值的生理相关范围（0 至 15 dyn/cm）内降低了约 45 mV。静电场在水/脂质界面附近呈现出峰值 (~0.8×109 V/m)，在 15 dyn/cm 处向双层中心移动 0.9 Å。 15 达因/厘米的最大膜张力导致每个脂质的面积增加 6.4%，双层厚度减少 4.7%，双层体积增加 1.4%。偶极电位敏感荧光探针用于检测暴露于渗透压的 DOPC 囊泡中膜张力诱导的变化。实验证实，DOPC 双层的偶极电位在膜张力较高时降低。这些结果暗示了一种潜在的新机械传感机制，通过该机制机械诱导脂质双层膜的结构变化可以通过改变静电相互作用和蛋白质构象状态的能量来调节膜蛋白的功能。
The dipole potential of lipid bilayer membrane controls the difference in permeability of the membrane to oppositely charged ions. We have combined molecular dynamics (MD) simulations and experimental studies to determine changes in electric field and electrostatic potential of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid bilayer in response to applied membrane tension. MD simulations based on CHARMM36 force field showed that electrostatic potential of DOPC bilayer decreases by ~45 mV in the physiologically relevant range of membrane tension values (0 to 15 dyn/cm). The electrostatic field exhibits a peak (~0.8×109 V/m) near the water/lipid interface which shifts by 0.9 Å towards the bilayer center at 15 dyn/cm. Maximum membrane tension of 15 dyn/cm caused 6.4% increase in area per lipid, 4.7% decrease in bilayer thickness and 1.4% increase in the volume of the bilayer. Dipole-potential sensitive fluorescent probes were used to detect membrane tension induced changes in DOPC vesicles exposed to osmotic stress. Experiments confirmed that dipole potential of DOPC bilayer decreases at higher membrane tensions. These results are suggestive of a potentially new mechanosensing mechanism by which mechanically induced structural changes in the lipid bilayer membrane could modulate the function of membrane proteins by altering electrostatic interactions and energetics of protein conformational states.
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影响因子：
3.4
作者：
FLEWELLING, RF;HUBBELL, WL
通讯作者：
HUBBELL, WL
影响因子：
3.4
作者：
Gullingsrud, J;Schulten, K
通讯作者：
Schulten, K
影响因子：
3.4
作者：
FRISCHLEDER, H;PEINEL, G
通讯作者：
PEINEL, G
影响因子：
4.4
作者：
FELLER, SE;ZHANG, YH;BROOKS, BR
通讯作者：
BROOKS, BR
DOI：
10.1016/0005-2736(75)90003-6
发表时间：
1975-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BLOK, MC;VANDERNEUTKOK, ECM;DEGIER, J
通讯作者：
DEGIER, J