TROAP Promotes Breast Cancer Proliferation and Metastasis
TROAP Promotes Breast Cancer Proliferation and Metastasis
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DOI：
10.1155/2019/6140951
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
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作者：
Li, Kai;Zhang, Ruo;Zhang, Lei
Trophinin相关蛋白(Troap)是微管细胞骨架调节和纺锤体组装所需的细胞质蛋白，其表达在多种肿瘤的发生发展中起关键作用。然而，人们对Troap在乳腺癌(BC)中的作用知之甚少。通过R2：基因组分析和可视化平台分析来自基因表达总集(GEO)数据集(GSE42568,104例BC患者；GSE1456,159例BC患者；GSE21653,266例BC患者)的Troap mRNA表达水平和临床数据，以评估总体生存(OS)。我们还通过基因本体论(GO)浓缩分析和京都基因与基因组百科全书(KEGG)分析了与Troap相关的基因，以预测Troap与BC中其他基因之间的潜在关系。我们的研究证实，在人BC样本和细胞系中，Troap的mRNA和蛋白表达水平都上调。体外实验表明，Troap基因敲除显著抑制了BC细胞的增殖、G1期向S期的转变以及BC细胞的迁移和侵袭能力。本研究提示，Troap在促进BC的增殖、侵袭和转移中起重要作用。
Trophinin-associated protein (TROAP) is a cytoplasmic protein required for microtubular cytoskeleton regulation and spindle assembly, and its expression plays a critical role in the initiation and progression of various types of cancer. However, little is known about the role of TROAP in breast cancer (BC). TROAP mRNA expression levels and clinical data from Gene Expression Omnibus (GEO) datasets (GSE42568, 104 BC patients; GSE1456, 159 BC patients; and GSE21653, 266 BC patients) were analyzed by the R2: Genomics Analysis and Visualization Platform to estimate overall survival (OS). We also analyzed the genes correlated with TROAP by gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to predict potential relationships between TROAP and other genes in BC. Our study verified that both TROAP mRNA and protein expression levels were upregulated in human BC samples and cell lines. In vitro experiments demonstrated that TROAP knockdown significantly inhibited cell proliferation, the G1 to S phase transition, and the migration and invasion abilities of BC cells. The present study suggests that TROAP plays an important role in promoting the proliferation, invasion, and metastasis of BC.