Epidemiology of rubella infection and genotyping of rubella virus in Cote d'Ivoire, 2012-2016
Epidemiology of rubella infection and genotyping of rubella virus in Cote d'Ivoire, 2012-2016
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DOI：
10.1002/jmv.25252
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发表时间：
2018-11-01
影响因子：
12.7
通讯作者：
Dosso, Mireille
中科院分区：
文献类型：
--
作者：
Kadjo, Herve A.;Waku-Kouomou, Diane;Dosso, Mireille
风疹是一种由风疹病毒（RuV）引起的传染性疾病，女性在怀孕早期感染风疹可导致严重的出生缺陷。本研究旨在描述科特迪瓦（CIV）风疹病毒的流行病学和遗传多样性。首先通过酶联免疫吸附测定 (ELISA) 检测从疑似麻疹病例采集的血液或口腔液样本中是否存在麻疹特异性 IgM 抗体。使用 ELISA 检测所有麻疹 IgM 阴性或不确定样本的风疹 IgM 抗体。通过实时逆转录聚合酶链反应 (RT-PCR) 检测风疹 IgM 阳性样本中是否存在风疹病毒 RNA。使用实时 RT-PCR 阳性 RNA 样本作为模板，扩增用于风疹基因分型的 739nt 区域。对 PCR 阳性样本进行测序并进行系统发育分析。 2012年至2016年间，通过麻疹监测系统收集了4121份血清和126份口腔液。其中，麻疹 IgM 阴性或不确定的分别为 3823 人和 108 人。随后的风疹检测发现，3823 份血清样本中的 690 份（18%）和 108 份口腔液样本中的 25 份（23%）风疹 IgM 呈阳性。对两份血清和七份口腔液样本的 E1 糖蛋白基因 739nt 片段进行了扩增和测序。系统发育分析表明，CIV风疹病毒属于基因型1G（8个样本）和2B（1个样本）基因型。2B基因型风疹病毒首次在CIV中发现。这些数据有助于提供风疹疫苗引入之前 CIV 中发现的风疹病毒株的基线信息。
Rubella is a contagious disease caused by the rubella virus (RuV) that can lead to serious birth defects when women are infected in early pregnancy. This study aimed to describe the epidemiology and genetic diversity of rubella viruses in Cote d'Ivoire (CIV). Blood or oral fluid samples collected from suspected measles cases were first tested for the presence of measles specific IgM antibodies by enzyme-linked immunosorbent assay (ELISA). All measles IgM negative or indeterminate samples were tested for rubella IgM antibody using ELISA. Rubella-IgM-positive samples were tested by real-time reverse transcription polymerase chain reaction (RT-PCR) for the presence of rubella virus RNA. Real-time RT-PCR-positive RNA samples were used as template to amplify the 739nt region used for rubella genotyping. PCR-positive samples were sequenced and phylogenetic analysis performed. Between 2012 and 2016, 4121 serums and 126 oral fluids were collected through the measles surveillance system. Of these, 3823 and 108 respectively were measles IgM negative or indeterminate. Subsequent testing for rubella found that 690 of 3823 (18%) serum samples and 25 of 108 (23%) oral fluid samples were rubella IgM-positive. The 739nt segment of the E1 glycoprotein gene was amplified and sequenced for two serums and seven oral fluids samples. Phylogenetic analysis showed that the rubella viruses from CIV belonged to genotypes 1G (eight samples) and 2B (one sample).Rubella virus genotype 2B was found in CIV for the first time. These data contribute to baseline information on rubella virus strains found in CIV before the introduction of rubella vaccine.