Pex16 is involved in peroxisome and Woronin body formation in the white koji fungus, Aspergillus luchuensis mut. kawachii
Pex16 is involved in peroxisome and Woronin body formation in the white koji fungus, Aspergillus luchuensis mut. kawachii
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DOI：
10.1016/j.jbiosc.2018.07.003
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发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Futagami, Taiki
中科院分区：
文献类型：
--
作者：
Kimoto, Daichi;Kadooka, Chihiro;Futagami, Taiki
本研究对禄劝曲霉突变株Pex 16进行了鉴定。kawachii研究了在烧酒发酵过程中过氧化物酶体对柠檬酸生产的作用。使用Delta pex 16菌株制成的米曲在柠檬酸积累方面没有显着变化，但甲酸产量增加了1.4倍。显微镜下观察mRFP-SKL（过氧化物酶体蛋白标记物）表明，pex 16破坏减少了每个菌丝细胞的点样结构的数量为对照的5%。Pex 16-GFP仅与mRFP-SKL在整个菌丝中共定位，包括非常靠近隔膜孔的位置。此外，Delta pex 16菌株对荧光钙白色高度敏感，荧光钙似乎诱导菌丝尖端破裂和mRFP-SKL信号移位到隔膜孔。这些结果表明，Pex 16不发挥作用，柠檬酸的积累，但显着参与过氧化物酶体和Woronin体的形成在白曲霉。(C)2018年，日本生物技术学会。All rights reserved.
We characterized Pex16 in Aspergillus luchuensis mut. kawachii to examine the role of peroxisomes on citric acid production during the shochu-fermentation process. Rice koji made using a Delta pex16 strain exhibited no significant change in citric acid accumulation but a 1.4-fold increase in formic acid production. Microscopic observation of mRFP-SKL (a peroxisome protein marker) showed that pex16 disruption decreased the number of dot-like structures per hyphal cell to 5% of the control. Pex16-GFP exclusively co-localized with mRFP-SKL throughout the hyphae including the very close position to the septal pore. Moreover, the Delta pex16 strain was hypersensitive to calcofluor white, which appeared to induce bursting of the hyphal tip and translocation of mRFP-SKL signals to the septal pore. These results indicate that Pex16 does not play a role in citric acid accumulation but is significantly involved in peroxisome and Woronin body formation in Aspergillus kawachii. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.