Small intestinal submucosa (SIS) in the repair of a cecal wound in unprepared bowel in rats
Small intestinal submucosa (SIS) in the repair of a cecal wound in unprepared bowel in rats
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DOI：
10.1007/s11605-007-0171-6
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Pappas, Theodore N.
中科院分区：
文献类型：
--
作者：
Ueno, Tomio;Oga, Atsunori;Pappas, Theodore N.
目的猪源性小肠黏膜下层（SIS）作为组织再生的脱细胞基质已被广泛接受。然而，其用于胃肠道缺损重塑的研究却很少。我们之前对啮齿动物胃的研究表明，SIS在酸性条件下刺激天然组织的再生。本研究的目的是探讨SIS作为生物支架用于无准备肠结肠缺损的可行性。方法在24只大鼠盲肠前壁切除1x1 cm的全层，用SIS修复。测量结果包括动物存活、原位补片稳定性以及3周和6个月时的组织学评估。结果大鼠体重明显增加，术后无明显渗漏。所有伤口均固定，并伴有网膜或其他脂肪粘连。组织学结果显示，术后6个月，所有病例的移植物区域均被完整的粘膜覆盖，缺损完全被肠壁的正常成分（粘膜、肌肉和神经细胞）所取代。结论SIS在促进未准备肠盲肠伤口愈合方面取得了很大的成功，并可作为原生结肠组织再生的生物支架。小肠粘膜下层在外科吻合中可能有助于促进愈合并可能防止渗漏。
Purpose Porcine-derived small intestinal submucosa (SIS) has been accepted as an acellular matrix for tissue regeneration. However, its use for remodeling gastrointestinal defects has been poorly investigated. Our previous study of the rodent stomach has demonstrated that the SIS stimulates regeneration of native tissue under acidic conditions. The purpose of this paper was to investigate the feasibility of using SIS as a bioscaffold for a colonic defect in unprepared bowel.Methods A 1x1-cm whole layer was excised on the anterior wall of the cecum in 24 rats, followed by onlay repair with SIS. Measurement outcomes included animal survival, mesh stability in situ, and histologic evaluation at 3 weeks and 6 months.Results Rats showed a significant weight gain and had no evidence of postoperative leakage. All wounds were secured and associated with either omental or other fatty adhesions. Histological findings revealed that intact mucosa covered the area of the graft in all cases 6 months after surgery and that the defect was completely replaced by the normal constituents (mucosa, muscle, and nerve cells) of the bowel wall.Conclusions SIS was largely successful in promoting healing in a cecal wound in unprepared bowel and serving as a bioscaffold for regeneration of the native colonic tissue. Small intestinal submucosa may be useful in surgical anastomoses to promote healing and presumably prevent leakage.