Analysis of sialoadhesin expression on mouse alveolar macrophages
Analysis of sialoadhesin expression on mouse alveolar macrophages
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DOI：
10.1016/j.imlet.2009.04.006
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发表时间：
2009-06-04
影响因子：
4.4
通讯作者：
Vanbever, Rita
中科院分区：
文献类型：
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作者：
Ducreux, Julie;Crocker, Paul R.;Vanbever, Rita
唾液酸粘附素（Sn）是一种巨噬细胞限制性受体，其首先在小鼠骨髓巨噬细胞上表征为介导绵羊红细胞结合（无需摄入）的受体。Sn在哺乳动物中高度保守，但其在组织巨噬细胞上的表达是异质的。在小鼠中，在淋巴组织的巨噬细胞上显示出高水平的红细胞结合，但在从支气管肺泡间隙分离的巨噬细胞上可检测到低红细胞结合活性。然而，Sn的表达已被证明对人类，大鼠和猪肺泡巨噬细胞（AM）使用分子生物学方法。因此，本研究旨在研究小鼠AM上Sn的表达，以确认该蛋白质在该鼠巨噬细胞群体上的存在。用细胞计数分析，我们表明，Sn的表达在小鼠AM表面。去唾液酸化后，AM大量结合红细胞，这种结合被抗小鼠Sn单克隆抗体3D6以剂量依赖性方式抑制。这表明Sn在小鼠AM上表达，但由该分子介导的唾液酸结合活性天然地被细胞表面上的糖萼内的内源性唾液酸掩蔽。(C)2009 Elsevier B.V.保留所有权利。
Sialoadhesin (Sn) is a macrophage-restricted receptor that was first characterised on mouse resident bone marrow macrophages as a receptor that mediates the binding, without ingestion, of sheep erythrocytes. Sn is highly conserved in mammals but its expression on tissue macrophages is heterogeneous. In the mouse, high levels of erythrocytes binding are shown on macrophages from lymphoid tissues but a low erythrocytes binding activity is detectable on macrophages isolated from the broncho-alveolar space. Yet, Sn expression has been demonstrated on human, rat and pig alveolar macrophages (AM) using methods of molecular biology. Therefore, the present study aimed to investigate the expression of Sn on mouse AM in order to confirm the presence of the protein on this population of murine macrophages. Using cytometrical analyses, we showed that Sn was expressed on mouse AM surface. Following desialyiation, AM largely bound erythrocytes and this binding was inhibited by 3D6, an anti-mouse Sn monoclonal antibody, in a dose-dependent manner. This indicates that Sn is expressed on mouse AM but that the sialic acid binding activity mediated by this molecule is naturally masked by endogenous sialic acid within the glycocalyx on the cell surface. (C) 2009 Elsevier B.V. All rights reserved.