Relationship between MALDI IMS Intensity and Measured Quantity of Selected Phospholipids in Rat Brain Sections
Relationship between MALDI IMS Intensity and Measured Quantity of Selected Phospholipids in Rat Brain Sections
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DOI：
10.1021/ac101079v
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发表时间：
2010-10-15
影响因子：
7.4
通讯作者：
Murphy, Robert C.
中科院分区：
文献类型：
--
作者：
Hankin, Joseph A.;Murphy, Robert C.
大鼠脑MALDI IMS正离子图像显示了一个区域分布的磷胆碱物种，这在这种描绘的明显独特性和可重复性方面是惊人的。这些图像的解释，特别是MALDI IMS离子强度与组织中磷酸胆碱（PC）物种数量之间的关系，受到许多因素的影响，如离子抑制、离子分子化学和组织结构的影响。本研究旨在验证PC分子种类的强度确实与组织样本中分子的数量有关的假设。采用LC/MS/MS技术，对有限但具有代表性的细胞源性PC分子种类进行了比较研究，测定了这些物种在脑组织提取物中的含量。LC/MS/MS分析的微解剖区PC分子种的MALDI IMS离子丰度与相应PC分子种的相对丰度具有良好的相关性。
MALDI IMS positive ion images of rat brain show a regional distribution of phosphocholine species that is striking in the apparent distinctiveness and reproducibility of such depictions. The interpretation of these images, specifically the relationship between MALDI IMS ion intensity and the amount of the phosphocholine (PC) species in the tissue is complicated by numerous factors, such as ion suppression, ion molecule chemistry, and effects of tissue structure. This study was designed to test the hypothesis that the intensity of PC molecular species does relate to the quantity of molecules in a tissue sample. A set of comparison studies for a limited but representative selection of cell-derived PC molecular species was carried out using LC/MS/MS to measure the amounts of these species in brain tissue extracts. There was good correlation between the MALDI IMS ion abundance of PC molecular species and the relative abundance of corresponding PC molecular species in microdissected regions analyzed by LC/MS/MS.