Structural characterization and anti-photoaging activity of a polysaccharide from Sargassum fusiforme
Structural characterization and anti-photoaging activity of a polysaccharide from Sargassum fusiforme
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DOI：
10.1016/j.foodres.2022.111267
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发表时间：
2022-04-23
影响因子：
8.1
通讯作者：
Hileuskaya, Kseniya
中科院分区：
文献类型：
--
作者：
Hu, Jinhong;Yao, Wanzi;Hileuskaya, Kseniya
本研究从羊栖菜中分离纯化了藻多糖（P1），并研究了其结构特征和抗光老化活性。结果表明，P1分子量为289 kDa，主要由甘露糖醛酸、古洛糖醛酸和岩藻糖组成，摩尔比为7.67:2.35:1.00。 P1 的主链为 -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 -> 4)-beta-ManA-(1 -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 ->)-beta-ManA-(1 -> 3,4)-beta-ManA-(1 -> 与 alpha-Fucp-(1 -> 末端基团连接)到-> 3,4)-beta-ManA-(1 ->的O-3位。此外，P1可以抑制UVB照射的HaCaT细胞中MMPs（MMP-1、MMP-3和MMP-9）的表达，表明P1可以减少UVB照射引起的胶原蛋白损失；还可以降低ROS和炎症因子（TNF-α、IL-6和IL-1β）的含量，表明P1可以减轻氧化应激和炎症因此，羊栖菜多糖P1可作为一种潜在的缓解皮肤光老化的功能食品。
In this study, a purified algal polysaccharide (P1) was isolated from Sargassum fusiforme and its structural characteristics and anti-photoaging activity were studied. Results showed that P1 had a molecular weight of 289 kDa and was mainly composed of mannuronic acid, guluronic acid and fucose with molar ratio of 7.67:2.35:1.00. The backbone of P1 was -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 -> 4)-beta-ManA-(1 -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 ->)-beta-ManA-(1 -> 3,4)-beta-ManA-(1 -> with a terminal group of alpha-Fucp-(1 -> linked to O-3 position of -> 3,4)-beta-ManA-(1 ->. In addition, P1 could inhibit the expressions of MMPs (MMP-1, MMP-3 and MMP-9) in the UVB-irradiated HaCaT cells, indicating that P1 could reduce collagen loss caused by UVB irradiation. It also reduced the contents of ROS and inflammatory factors (TNF-alpha, IL-6 and IL-1 beta), indicating that P1 could reduce the oxidative stress and inflammation response. Thus, Sargassum fusiforme polysaccharide P1 could be used as a potential functional food to relieve skin photoaging.