Enumeration of haemopoietic progenitors (CFU-C and BFU-E) in liquid microculture using a limiting dilution analysis.
Enumeration of haemopoietic progenitors (CFU-C and BFU-E) in liquid microculture using a limiting dilution analysis.
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使用有限稀释分析对液体微培养物中的造血祖细胞（CFU-C 和 BFU-E）进行计数。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
G. Milon
中科院分区：
文献类型：
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作者：
G. Marchal;G. Milon
为了克服对造血祖细胞的多中心集落进行评分的困难，使培养物在10微升液体培养基中生长。在三种不同的细胞浓度下铺板，祖细胞（CFU-C或BFU-E）不存在于所有威尔斯孔中。孵育7天后，在微量培养物的每个孔中很容易对它们的存在进行评分。有限稀释分析允许获得准确的定量。
In order to overcome the difficulty of scoring multicentric colonies of haemopoietic progenitor cells, cultures in 10 microliter of liquid medium were grown. Plated at three different cell concentrations, progenitor cells (CFU-C or BFU-E) are not present in all wells. Their presence is easily scored in each well of microculture 7 days after incubation. A limiting dilution analysis allows one to obtain accurate quantification.