Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes
Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes
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DOI：
10.1093/nar/24.22.4501
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发表时间：
1996-11-15
影响因子：
14.9
通讯作者：
Honda, K
中科院分区：
文献类型：
--
作者：
Sugimoto, N;Nakano, S;Honda, K
To improve the previous DNA/DNA nearest-neighbor parameters, thermodynamic parameters (Delta H degrees, Delta S degrees and Delta G degrees) of 50 DNA/DNA duplexes were measured. Enthalpy change of a helix initiation factor is also considered though the parameters reported recently did not contain the factor. A helix initiation factor for DNA/DNA duplex determined here was the same as that of RNA/RNA duplex (Delta G degrees(37) = 3.4 kcal/mol). The improved nearest-neighbor parameters reproduced not only these 50 experimental values used here but also 15 other experimental values obtained in different studies. Comparing Delta G degrees(37) values of DNA/DNA nearest-neighbor parameters obtained here with those of RNA/RNA and RNA/DNA, RNA/RNA duplex was generally the most stable of the three kinds of duplexes with the same nearest-neighbor sequences. Which is more stable between DNA/DNA and RNA/DNA duplexes is sequence dependent.
