Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions
Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions
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DOI：
10.1093/pcp/pcn144
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Barroso, Juan B.
中科院分区：
文献类型：
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作者：
Corpas, Francisco J.;Chaki, Mounira;Barroso, Juan B.
一氧化氮（NO）是动植物不同生理过程中的重要信号分子。然而，在非生物胁迫条件下，植物内源NO和其他活性氮（RNS）的代谢却知之甚少。使用暴露于六种不同非生物胁迫条件下的豌豆植物（强光、低温和高温、连续光照、连续黑暗和机械创伤）、RNS代谢的几个关键组分包括NO、S-亚硝基硫醇（RSNOs）和亚硝酸盐和硝酸盐的含量、烟碱依赖的一氧化氮合酶（NOS）和S-亚硝基谷胱甘肽还原酶（GSNOR）的酶活性、和蛋白质酪氨酸硝化（NO_2-Tyr）谱。低温是应激，产生的NOS和GSNOR活性的增加最高，这是伴随着总NO和S-亚硝基硫醇的含量增加，并加强与NO2-Tyr抗体的免疫反应。机械伤害、高温和光照对豌豆RNS代谢的不同指标也有明显的激活作用。然而，在叶片中的亚硝酸盐和硝酸盐的总含量没有受到任何这些压力。考虑到蛋白质酪氨酸硝化是亚硝化胁迫的一个潜在标记，所获得的结果表明，低温和高温，连续光照和高光强的非生物胁迫条件下，可以诱导豌豆亚硝化胁迫。
Nitric oxide (NO) is a key signaling molecule in different physiological processes of animals and plants. However, little is known about the metabolism of endogenous NO and other reactive nitrogen species (RNS) in plants under abiotic stress conditions. Using pea plants exposed to six different abiotic stress conditions (high light intensity, low and high temperature, continuous light, continuous dark and mechanical wounding), several key components of the metabolism of RNS including the content of NO, S-nitrosothiols (RSNOs) and nitrite plus nitrate, the enzyme activities of arginine-dependent nitric oxide synthase (NOS) and S-nitrosogluthathione reductase (GSNOR), and the profile of protein tyrosine nitration (NO2-Tyr) were analyzed in leaves. Low temperature was the stress that produced the highest increase of NOS and GSNOR activities, and this was accompanied by an increase in the content of total NO and S-nitrosothiols, and an intensification of the immunoreactivity with an antibody against NO2-Tyr. Mechanical wounding, high temperature and light also had a clear activating effect on the different indicators of RNS metabolism in pea plants. However, the total content of nitrite and nitrate in leaves was not affected by any of these stresses. Considering that protein tyrosine nitration is a potential marker of nitrosative stress, the results obtained suggest that low and high temperature, continuous light and high light intensity are abiotic stress conditions that can induce nitrosative stress in pea plants.