A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells
A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells
复制标题
DOI：
10.1159/000273266
复制
发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Raposio, E.
中科院分区：
文献类型：
--
作者：
Coradeghini, R.;Guida, C.;Raposio, E.
人脂肪干细胞具有多种干细胞特性，可作为干细胞群体的来源。在此基础上，我们研究了脂肪来源的干细胞的肝潜能，从吸脂术中获得，以下两个分化方案。在第一个程序中，培养基中补充有表皮生长因子（EGF），碱性成纤维细胞生长因子，肝细胞生长因子（HGF）和烟酰胺;第二个涉及添加因子，如地塞米松，EGF，胰岛素-转铁蛋白-亚硒酸钠，HGF，二甲基亚砜和制瘤素。同时，我们在Hep G2细胞系中进行了我们的研究，作为人肝分化的体外模型。免疫细胞化学分析和RT-PCR检测肝细胞分化情况。采用DNA含量、MTT比色法和羧基荧光素琥珀酰亚胺酯染色法检测细胞增殖情况。我们报道了在未分化脂肪干细胞中存在基础肝标志物的证据，证实了其多能性。在肝诱导的脂肪源性干细胞分化过程中，观察到白蛋白和甲胎蛋白的强表达。两种类型的肝脂肪源性干细胞的形态学方面是相似的。增殖指数表明，第一分化程序比第二促进生长。这些初步的发现表明脂肪来源的干细胞可以被诱导成肝谱系，并且两种标准分化程序之间最显著的差异在于增殖率。当脂肪干细胞用于研究和临床研究时，这方面是要考虑的。版权所有（C）2009 S. Karger AG，巴塞尔
Human adipose-derived stem cells possess a lot of stem cell characteristics, so they may be considered a source of stem cell population. On the basis of that, we have investigated the hepatic potential of adipose-derived stem cells, obtained from liposuction, following two differentiation protocols. In the first procedure, medium was supplemented with epidermal growth factor (EGF), basic fibroblast growth factor, hepatocyte growth factor (HGF) and nicotinamide; the second involved the addition of factors such as dexametasone, EGF, insulin-transferrin-sodium selenite, HGF, dimethyl sulfoxide and oncostatin. In parallel, we carried out our study in the Hep G2 cell line, as human hepatic differentiated in vitro model. Immunocytochemical analysis and RT-PCR were performed using hepatic markers to evaluate cell differenttiation. DNA content, MTT test and carboxyl fluorescein succinimidyl ester staining were carried out to evaluate cell proliferation. We reported the evidence of basal hepatic marker in undifferentiated adipose-derived stem cells, which confirmed their multipotency. A strong expression of albumin and alpha-fetoprotein was observed in hepatic-induced adipose-derived stem cells following both differentiation procedures. Morphological aspects of the two types of hepatic adipose-derived stem cells were alike. Proliferation index suggested that the first differentiation procedure promoted better growth than the second. These preliminary findings suggest adipose-derived stem cells may be induced into hepatic lineage, and the most significant difference between the two standard differentiation procedures concerns proliferation rate. This aspect is to be considered when adipose-derived stem cells are employed in research and clinical studies. Copyright (C) 2009 S. Karger AG, Basel