Evaluation of in vitro reconstituted basement membrane assay to assess the invasiveness of tumor cells.
Evaluation of in vitro reconstituted basement membrane assay to assess the invasiveness of tumor cells.
复制标题
体外重建基底膜测定评估肿瘤细胞的侵袭性。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
J. Foidart
中科院分区：
文献类型：
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作者：
N. Simon;Agnès Noël;J. Foidart
肿瘤细胞穿过基底膜是转移过程中的关键步骤。我们在Boyden室中使用覆盖在过滤器上的重建基底膜（基质胶）来评估肿瘤和正常细胞的侵袭潜力。未发现体外化学侵袭与体内转移潜能之间的相关性。正常人成纤维细胞和鼠3 T3成纤维细胞穿透用基质胶包被的滤器。另一方面，肿瘤细胞（MCF 7、MCF 7 gpt、MCF 7 ras、BeWo、NH 4、NUC-1细胞）不能穿过基质。我们的研究结果表明，在我们的条件下，这种广泛使用的模型来评估肿瘤的侵袭并没有提供一个通用的检测方法来测试肿瘤细胞的侵袭性。基质胶的渗透似乎与取决于细胞类型的趋化或趋触反应有关。我们的数据强调了与基底膜侵袭相关的分子事件的可变性。
The crossing of tumor cells through basement membranes represents a critical step in the metastatic process. We have used a reconstituted basement membrane (matrigel) coated on filter in a Boyden chamber to assess the invasive potential of tumor and normal cells. No correlation was found between chemoinvasion in vitro and the metastatic potential in vivo. Normal human fibroblasts and murine 3T3 fibroblasts penetrated filters coated with matrigel. On the other hand, the tumoral cells (MCF7, MCF7 gpt, MCF7 ras, BeWo, JAR, NUC-1 cells) were unable to cross the matrix. Our results suggest that in our conditions, this widely used model to assess tumoral invasion does not provide a universal assay to test the invasiveness of tumor cells. Penetration of the matrigel appears to be related to chemotactic or haptotactic responses depending upon cell types. Our data emphasize the variability of molecular events associated with basement membrane invasion.