Identification and molecular characterization of BP75, a novel bromodomain-containing protein
Identification and molecular characterization of BP75, a novel bromodomain-containing protein
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DOI：
10.1016/s0014-5793(99)01191-6
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发表时间：
1999-10-15
期刊：
影响因子：
3.5
通讯作者：
Hendriks, W
中科院分区：
文献类型：
--
作者：
Cuppen, E;van Ham, M;Hendriks, W
我们在此描述了一种新型含布罗莫结构域蛋白，即 75 kDa 的布罗莫结构域蛋白 (BP75)。最初，我们通过双杂交筛选与蛋白酪氨酸中第一个 PDZ（突触后密度蛋白 PSD-95、果蝇盘大肿瘤抑制蛋白 D1gA 和紧密连接蛋白 ZO-1 的缩写）相互作用的蛋白，鉴定了 BP75。磷酸酶-BAS 样（PTP-BL），我们发现 BP75 普遍表达，并表明 BP75 和由第一个 PDZ 结构域组成的 PTP-BL 缺失突变体主要位于细胞核中，尽管细胞质定位也很明显。相反，全长 PTP-BL 主要位于细胞质中，尽管观察到一些基础核染色。所描述的分子相互作用可能反映了膜下信号传导事件和核事件的耦合机制。 (C) 1999 年欧洲生化学会联合会。
We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75), Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor D1gA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL), We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events. (C) 1999 Federation of European Biochemical Societies.