Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis
Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis
复制标题
DOI：
10.1038/s41477-024-01666-3
复制
发表时间：
2024-04-08
期刊：
影响因子：
18
通讯作者：
Cole，Benjamin
中科院分区：
文献类型：
--
作者：
Serrano，Karen;Bezrutczyk，Margaret;Cole，Benjamin
植物与丛枝菌根真菌（AM）的共生相互作用是古老而广泛的。植物为AM真菌提供碳以交换营养和水，使这种相互作用成为作物改良的主要目标。然而，植物-真菌相互作用仅限于根细胞的一小部分，排除了大多数常规功能基因组技术的应用，以研究这些相互作用的分子基础。在这里，我们使用单核和空间RNA测序，探讨无论是苜蓿和Rhizophagus irregularisetranscriptomes在AM共生在细胞和空间分辨率。综合，空间注册的单细胞图显示感染和未感染的植物根细胞类型。我们观察到，皮层细胞表现出不同的转录组配置文件在不同阶段的殖民AM真菌，表明动态的相互作用，在建立细胞界面，使成功的共生过程中，这两种生物体之间。我们的研究深入了解了具有重大农业和环境重要性的共生关系，并展示了结合单细胞和空间转录组学来分析复杂生物相互作用的范式。
The symbiotic interaction of plants with arbuscular mycorrhizal (AM) fungi is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant–fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. Here we used single-nucleus and spatial RNA sequencing to explore bothMedicago truncatulaandRhizophagus irregularistranscriptomes in AM symbiosis at cellular and spatial resolution. Integrated, spatially registered single-cell maps revealed infected and uninfected plant root cell types. We observed that cortex cells exhibit distinct transcriptome profiles during different stages of colonization by AM fungi, indicating dynamic interplay between both organisms during establishment of the cellular interface enabling successful symbiosis. Our study provides insight into a symbiotic relationship of major agricultural and environmental importance and demonstrates a paradigm combining single-cell and spatial transcriptomics for the analysis of complex organismal interactions.