HIGH-FREQUENCY SONOPHORESIS - PERMEATION PATHWAYS AND STRUCTURAL BASIS FOR ENHANCED PERMEABILITY
HIGH-FREQUENCY SONOPHORESIS - PERMEATION PATHWAYS AND STRUCTURAL BASIS FOR ENHANCED PERMEABILITY
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DOI：
10.1159/000211287
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发表时间：
1994-05-01
期刊：
影响因子：
--
通讯作者：
ELIAS, PM
中科院分区：
文献类型：
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作者：
MENON, GK;BOMMANNAN, DB;ELIAS, PM
超声波促渗角质层的机制尚不清楚。我们在这里研究了渗透途径，和SC细胞间的结构组织应用高频超声导入无毛小鼠皮肤。钌四氧化物后固定和示踪溶液的硝酸镧和FITC-葡聚糖被用来检查SC层状双层，层状体形态和亚细胞渗透途径。超声波电泳破坏了颗粒层（SG）-SC界面处SC双层和LB衍生内容物的紧密组织，导致0和20 h之间的结构域分离，48 h恢复。超声促渗后，示踪剂通过层状双层内的腔隙穿过SC，并通过层状结构显示域分离的位点。这些研究提供了有关渗透途径，透化机制和动力学的表皮上的超声导入的见解。
The mechanism of stratum corneum (SC) permeabilization by ultrasound (sonophoresis) is unknown. We studied here permeation pathways, and SC intercellular structural organization following applications of high-frequency sonophoresis to hairless mouse skin. Ruthenium tetroxide post-fixation and tracer solutions of LaNO3 and FITC-dextrans were employed to examine SC lamellar bilayers, lamellar body morphology and subcellular permeation pathways. Sonophoresis disrupted the compact organization of SC bilayers and LB-derived contents at the stratum granulosum (SG)-SC interface, leading to domain separation between 0 and 20 h, reverting by 48 h. Post-sonophoresis, tracers traversed the SC via lacunae within the lamellar bilayers, and via lamellae in sites that displayed domain separation. These studies provide insights about the penetration pathways, permeabilizing mechanisms, and kinetics of sonophoresis on the epidermis.