Roles of T-cells in the development of allergic conjunctival diseases.
Roles of T-cells in the development of allergic conjunctival diseases.
复制标题
T 细胞在过敏性结膜疾病发展中的作用。
DOI：
10.1097/ico.0b013e31812f6970
复制
发表时间：
2007
期刊：
影响因子：
2.8
通讯作者：
A. Fukushima
中科院分区：
文献类型：
--
作者：
A. Fukushima
大量嗜酸性粒细胞浸润是严重形式的过敏性结膜疾病（ACD）如春季角结膜炎的典型组织病理学特征。虽然ACD是抗原（Ag）特异性疾病，但嗜酸性粒细胞缺乏Ag特异性受体。因此，我的小组研究了携带Ag特异性受体的免疫活性细胞在ACD中诱导结膜嗜酸性粒细胞浸润的可能作用。为了诱导实验性结膜炎（EC），通过转移Ag特异性或引发的IgE、脾细胞或纯化的T细胞对Brown Norway大鼠和C57 BL/6和Balb/c小鼠进行被动免疫，然后用滴眼液中的Ag进行攻击。Ag-致敏的脾细胞或T细胞的转移，而不是Ag-特异性IgE，诱导结膜嗜酸性粒细胞浸润。我们还表明，EC诱导后不久，T细胞转移，活化的Ag特异性T细胞浸润结膜。此外，当EC发育动物局部治疗的T细胞特异性免疫抑制剂环孢素A和FK 506，结膜嗜酸性粒细胞浸润被废除。此外，只有Ag特异性2型辅助T细胞（TH 2细胞）的转移，而不是1型辅助T细胞或CD 8 T细胞，可以诱导结膜嗜酸性粒细胞浸润。我们的数据表明，Ag特异性TH 2细胞在EC发展过程中诱导结膜嗜酸性粒细胞浸润中起着至关重要的作用。有必要阐明结膜上皮细胞和免疫活性细胞在EC结膜嗜酸性粒细胞浸润中的作用。
Massive infiltration of eosinophils is the typical histopathologic feature of severe forms of allergic conjunctival diseases (ACDs) such as vernal keratoconjunctivitis. Although ACD is an antigen (Ag)-specific disease, eosinophils lack Ag-specific receptors. Therefore, my group studied the likely roles of immunocompetent cells bearing Ag-specific receptors in inducing conjunctival eosinophil infiltration in ACDs. To induce experimental conjunctivitis (EC), Brown Norway rats and C57BL/6 and Balb/c mice were passively immunized by the transfer of Ag-specific or primed IgE, splenocytes, or purified T-cells and then challenged with Ag in eyedrops. The transfer of Ag-primed splenocytes or T-cells, but not of Ag-specific IgE, induced conjunctival eosinophil infiltration. We also showed that soon after EC induction by T-cell transfer, activated Ag-specific T-cells infiltrated the conjunctiva. Moreover, when EC-developing animals were topically treated with the T-cell-specific immunosuppressants cyclosporin A and FK506, conjunctival eosinophil infiltration was abrogated. In addition, only the transfer of Ag-specific type 2 helper T-cells (TH2 cells), but not of type 1 helper T-cells or CD8 T-cells, could induce conjunctival eosinophil infiltration. Our data show that Ag-specific TH2 cells play a crucial role in inducing conjunctival eosinophil infiltration during the development of EC. It is necessary to elucidate the roles that conjunctival residential cells and immunocompetent cells other than T-cells play in the conjunctival eosinophil infiltration of EC.
CD8 T 细胞在小鼠实验性过敏性结膜炎的诱导和效应阶段中发挥不同的作用
DOI：
--
发表时间：
2006
期刊：
Microbiol Immunol 50(9)
影响因子：
--
作者：
Ueda;S. et al.;Inoue J.;Fukushima A
通讯作者：
Fukushima A
影响因子：
4.4
作者：
Ozaki, A;Seki, Y;Kubo, M
通讯作者：
Kubo, M