L-amino acid oxidase 1 in sperm is associated with reproductive performance in male mice and bulls
L-amino acid oxidase 1 in sperm is associated with reproductive performance in male mice and bulls
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DOI：
10.1093/biolre/ioab024
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发表时间：
2021-02-16
影响因子：
3.6
通讯作者：
Nagaoka，Kentaro
中科院分区：
文献类型：
--
作者：
Zhang，Haolin;Liu，Hong;Nagaoka，Kentaro
精子质量是男性生育能力的重要指标，合适的生物标志物可以选择高质量的精子。我们之前发现由l -氨基酸氧化酶1 （Lao1）基因编码的l -氨基酸氧化酶在乳腺和大脑中发挥生物学作用，将特定的l -氨基酸转化为酮酸、氨和过氧化氢（H2O2）。在这里，我们描述了flao1在男性生殖中的作用。Lao1缺陷（Lao1−/−）的雄性小鼠产生的怀孕胚胎和幼崽较少，受精卵比例较低，甚至排卵数量也没有差异，这表明雄性低生育能力导致了小窝。我们发现在顶体中表达的LAO1与高畸形率和低存活率的LAO1−/−精子有关。野生型（WT）精子产生的h2o2比la1−/−精子多，10 μM h2o2在体外恢复了敲除（KO）精子的活力。此外，WT诱导顶体反应的精子比例高于用钙离子载体A23187孵育的la1−/−精子。在高受精率的牛精子中，LAO1表达丰富。我们认为，定位于精子顶体的LAO1影响精子的活力和形态以及顶体反应，LAO1缺失的精子可能导致男性不育。因此，la1可能作为一种新的标记物，用于选择优质精子，特别是牲畜生殖。
Sperm quality is an important indicator of male fertility, and a suitable biomarker enables the selection of high-quality spermatozoa. We previously found that L-amino acid oxidase encoded by the L-amino acid oxidase 1 (Lao1) gene exerts biological roles in the mammary gland and brain by converting specific L-amino acids into keto acids, ammonia, and hydrogen peroxide (H2O2). Here, we describe the role ofLao1in male reproduction.Lao1-deficient (Lao1−/−) male mice generated fewer pregnant embryos and pups as well as lower ratios of fertilized oocytes and even ovulated number was not different, suggesting that male subfertility caused the smaller litters. We found that LAO1 expressed in acrosomes is associated with high malformation ratios and low viability ofLao1−/−sperm. Wild-type (WT) sperm produced more H2O2thanLao1−/−sperm, and 10 μM H2O2restored knockout (KO) sperm viability in vitro. In addition, the sperm ratio of induced acrosome reaction was higher in WT than inLao1−/−sperm incubated with the calcium ionophore A23187. Moreover, LAO1 expression was abundant in bovine sperm with high fertilization ratios. We concluded that LAO1 localized in the sperm acrosome influences sperm viability and morphology as well as the acrosome reaction, and thatLAO1-deficient sperm might cause male subfertility. Thus, LAO1 might serve as a novel marker for selecting high-quality spermatozoa, especially for livestock reproduction.