Increased C-type natriuretic peptide mRNA expression in the kidney of diabetic rats.
Increased C-type natriuretic peptide mRNA expression in the kidney of diabetic rats.
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DOI：
10.1677/joe.0.1580035
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发表时间：
1998-07
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影响因子：
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通讯作者：
S. Shin;J. Wen;Y. J. Lee;I. Chen;J. Tsai
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文献类型：
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作者：
S. Shin;J. Wen;Y. J. Lee;I. Chen;J. Tsai
为了研究肾脏合成的C型利钠肽（CNP）对水和电解质平衡变化的反应性，我们测定了链脲佐菌素诱导的糖尿病大鼠进食正常（0.26%NaCl）或低盐（0.04%NaCl）饮食时肾脏CNP mRNA水平、血浆CNP浓度和尿CNP排泄率。使用逆转录-PCR，然后Southern印迹分析，我们发现，肾皮质和髓质CNP mRNA水平显着增强糖尿病大鼠从第14天，并保持增强与伴随的升高尿CNP排泄率为整个60天的研究期间。糖尿病大鼠肾脏CNP mRNA和尿CNP排泄率的增加在低盐饮食治疗的糖尿病大鼠和胰岛素治疗的糖尿病大鼠中均被减弱。这些结果表明，在糖尿病大鼠中，肾脏CNP合成增强，并且肾脏CNP mRNA的增加通过盐限制和胰岛素治疗而得到改善。这些结果表明，肾脏合成的CNP对糖尿病大鼠水和电解质稳态的改变有反应。
To investigate the responsiveness of renal-synthesized C-type natriuretic peptide (CNP) to changes in water and electrolyte balance, we measured renal CNP mRNA levels, plasma CNP concentrations and urinary CNP excretion rates in streptozotocin-induced diabetic rats eating a normal (0.26% NaCl) or low (0.04% NaCl) salt diet. Using reverse transcription-PCR followed by Southern blot analysis, we found that renal cortical and medullary CNP mRNA levels were markedly enhanced in diabetic rats from the 14th day and remained enhanced with an accompanying elevation of urinary CNP excretion rates for the entire 60-day study period. All increases of renal CNP mRNA and urinary CNP excretion rates in diabetic rats were attenuated in low salt diet-treated diabetic rats as well as insulin-treated diabetic rats. These results demonstrate that renal CNP synthesis is enhanced in diabetic rats and the increase of renal CNP mRNA is ameliorated by salt restriction and insulin treatment. These results imply that renal-synthesized CNP is responsive to the alteration of water and electrolyte homeostasis in diabetic rats.