Response of seasonally cold-water bacterioplankton to temperature and substrate treatments
Response of seasonally cold-water bacterioplankton to temperature and substrate treatments
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DOI：
10.1006/ecss.1997.0282
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发表时间：
1998-04-01
影响因子：
2.8
通讯作者：
Autio, R
中科院分区：
文献类型：
--
作者：
Autio, R
通过3个间歇培养实验，研究了冷水浮游细菌对温度和基质处理的响应。实验是在1989年和1991年使用预过滤的波罗的海海水。将原位水温（+2 ° C）增加10 ° C，并使用蔗糖作为碳源。实验进行6-9天。测量胸苷掺入、细胞数量和体积。生长速率和胸苷转化因子进行了估计。所研究的细菌群落的影响，通过温度的操纵和提高温度和底物添加的综合效应。在较高的温度下，胸苷掺入和细胞数量增加。另一方面，单独的底物处理不能改变细菌群落的生长模式。因此，不稳定的碳源，在添加水平，在这些实验中的冷水浮游细菌的调节中不是最关键的因素。只有当实验温度高于原位温度时，细菌才能够利用添加的蔗糖。然而，仅较高的温度就能使细菌使用其他天然的、可能更复杂的物质作为碳源。(C)出版社：Academic Press Limited。
Cold-water bacterioplankton response to temperature and substrate treatments was studied in three brackish-water batch-culture experiments. The experiments were done during 1989 and 1991 using prefiltrated Baltic Sea water. The in situ water temperature (+2 degrees C) was increased by 10 degrees C, and sucrose was used as a carbon source. The experiments were run for 6-9 days. Thymidine incorporation, cell numbers and volumes were measured. Growth rates and thymidine conversion factors were estimated.The studied bacterial community was affected by the temperature manipulation and by the combined effect of increased temperature and substrate addition. At the higher temperature both thymidine incorporation and cell numbers were increased. On the other hand, substrate treatment alone was not able to change the growth pattern of the bacterial community. Thus, the labile carbon source, at the addition level, was not the most critical factor in the regulation of cold-water bacterioplankton in these experiments. The bacteria were able to take advantage of the added sucrose only when the experimental temperature was higher than in situ. Higher temperature alone, however, enabled bacteria to use other natural, probably more complicated, substances as their source of carbon. (C) 1998 Academic Press Limited.