Malignancies of the uterine corpus and immunoreactivity score of the DNA "mismatch-repair" enzyme human Mut-S-Homologon-2
Malignancies of the uterine corpus and immunoreactivity score of the DNA "mismatch-repair" enzyme human Mut-S-Homologon-2
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DOI：
10.1177/002215549904700112
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发表时间：
1999-01-01
影响因子：
3.2
通讯作者：
Reichrath, J
中科院分区：
文献类型：
--
作者：
Friedrich, M;Villena-Heinsen, C;Reichrath, J
我们分析了人类Mut-S同源基因-2在正常子宫内膜组织(n=15)和子宫体恶性肿瘤组织(n=40)中的表达。用高灵敏的链霉亲和素-过氧化物酶技术和鼠特异性单抗(FE11克隆)，对冰冻切片上的人Mut-S同源蛋白进行了免疫组织化学研究。将人Mut-S-Homologon-2标记模式与增殖标记物Ki-67在同一肿瘤中的染色模式进行比较。本文提出了一种半定量分析人Mut-S-Homologon-2表达的免疫反应评分(人Mut-S-Homologon-2-IRS：阴性0-1；弱2-3；中等4-6；强8-12)。在正常子宫内膜组织中，67%的人Mut-S-Homologon-2呈弱阳性反应，而其余33%的人Mut-S-Homologon-2均为阴性(平均值为Mut-S-Homologon-2-IRS1.25+/-1.29)。分析的子宫体癌显示中到强的核免疫反应(平均人Mut-S-Homologon-2-IRS9.00，+/-3.16)。人Mut-S-Homologon-2染色是异质性的，单个肿瘤细胞之间存在视觉差异。与正常子宫内膜组织相比，人Mut-S-Homologon-2蛋白在子宫肌体恶性肿瘤细胞中的表达持续且强烈上调(人Mut-S-Homologon-2-PP p<；0.001；人Mut-S-Homologon-2-is p
We analyzed human Mut-S-Homologon-2 expression in normal endometrial tissue (n = 15) and malignancies of the uterine corpus (n = 40). Human Mut-S-Homologon-2 protein was investigated immunohistochemically on frozen sections, using a highly sensitive streptavidin-peroxidase technique and a specific mouse monoclonal antibody (clone FE11). Human Mut-S-Homologon-2 labeling pattern was compared with the staining pattern of the proliferation marker Ki-67 in the same tumors. A human Mut-S-Homologon-2 immunoreactivity score (human Mut-S-Homologon-2-IRS: negative 0-1; weak 2-3; moderate 4-6; strong 8-12) for semiquantitative analysis of human Mut-S-Homologon-2 expression is presented. In normal endometrial tissue samples we found weak nuclear immunoreactivity for human Mut-S-Homologon-2 in 67%, whereas the remaining 33% were negative for human Mut-S-Homologon-2 (mean hu man Mut-S-Homologon-2-IRS 1.25 +/- 1.29). Al I malignancies of the uterine corpus analyzed revealed moderate to strong nuclear immunoreactivity (mean human Mut-S-Homologon-2-IRS 9.00, +/- 3.16). Human Mut-S-Homologon-2 staining was heterogeneous, with Visual differences among individual tumor cells. Expression of human Mut-S-Homologon-2 protein was consistently and strongly upregulated in tumor cells of malignancies of the uterine corpus compared with normal endometrial tissue (human Mut-S-Homologon-2-PP p < 0.001; human Mut-S-Homologon-2-IS p