Spectral light measurements in microbenthic phototrophic communities with a fiber‐optic microprobe coupled to a sensitive diode array detector
Spectral light measurements in microbenthic phototrophic communities with a fiber‐optic microprobe coupled to a sensitive diode array detector
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使用与灵敏二极管阵列探测器耦合的光纤微探针对微底栖光养群落进行光谱光测量
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发表时间：
1992
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通讯作者：
B. Jørgensen
中科院分区：
文献类型：
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作者：
M. Kühl;B. Jørgensen
开发了一种用于光纤微探针的微尺度光测量的二极管阵列检测器系统;它测量400-900 nm光的强度超过6个数量级，光谱分辨率为2-5 nm。光纤微探针测量场辐射或标量辐照度耦合到检测器系统，并用于光谱光测量在高盐微生物垫和沿海沉积物的层状光养群落。微藻、蓝细菌和不产氧光合细菌的主要色素的垂直分布可以通过在430-550 nm（Chl a和类胡萝卜素）、620-625 nm（藻蓝蛋白）、675 nm（Chl a）、745-750 nm（BChl c）、800-810 nm和860-880 nm（BChl a）处测量的辐射光谱中的消光最大值来确定。标量辐射光谱表现出不同的光谱组成和更高的光强度在沉积物表面相比，入射光。因此，IR光在沉积物表面处达到200%的入射。最大的光穿透被发现为IR光，而可见光强烈衰减在上0-2毫米的沉积物。光子标量辐照度（400-700 nm）的测量结合微电极测量沿海沉积物中的产氧光合作用。当入射光强度为200 [mu]Einst m[sup [minus]2]s[sup [minus]1]时，光子标量更多»辐照度在沉积物表面达到最大值283 [mu]Einst m[sup [minus]2]s[sup [minus]1]。在12 [mu]Einst m[sup [minus]2]s[sup [minus]1]的光强度下，真光区的下边界为表面以下2.2 mm。参考文献20篇，图6。«少
A diode array detector system for microscale light measurements with fiber-optic microprobes was developed; it measures intensities of 400-900-nm light over >6 orders of magnitude with a spectral resolution of 2-5 nm. Fiber-optic microprobes to measure field radiance or scalar irradiance were coupled to the detector system and used for spectral light measurements in hypersaline microbial mats and in laminated phototrophic communities of coastal sediments. The vertical distribution of major photopigments of microalgae, cyanobacteria, and anoxygenic phototrophic bacteria could be identified from extinction maxima in measured radiance spectra at 430-550 nm (Chl a and carotenoids), 620-625 nm (phycocyanin), 675 nm (Chl a), 745-750 nm (BChl c), 800-810 nm, and 860-880 nm (BChl a). Scalar irradiance spectra exhibited a different spectral composition and a higher light intensity at the sediment surface as compared to incident light. IR light thus reached 200% of incident at the sediment surface. Maximal light penetration was found for IR light, whereas visible light was strongly attenuated in the upper 0-2 mm of the sediment. Measurements of photon scalar irradiance (400-700 nm) were combined with microelectrode measurements of oxygenic photosynthesis in the coastal sediment. With an incident light intensity of 200 [mu]Einst m[sup [minus]2]s[sup [minus]1], photon scalarmore » irradiance reached a maximum of 283 [mu]Einst m[sup [minus]2]s[sup [minus]1] at the sediment surface. The lower boundary of the euphotic zone was 2.2 mm below the surface at a light intensity of 12 [mu]Einst m[sup [minus]2]s[sup [minus]1]. 20 refs., 6 figs.« less