Unique temperature-sensitive defect in vaccinia virus morphogenesis maps to a single nucleotide substitution in the A30L gene.
Unique temperature-sensitive defect in vaccinia virus morphogenesis maps to a single nucleotide substitution in the A30L gene.
复制标题
痘苗病毒形态发生中独特的温度敏感缺陷归因于 A30L 基因中的单核苷酸取代。
DOI：
10.1128/jvi.75.22.11222-11226.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Moss,B
中科院分区：
文献类型：
--
作者：
Szajner,P;Weisberg,AS;Moss,B
标记拯救实验表明，先前分离的痘苗病毒温度敏感突变体的遗传损伤形成具有透明内部的多层包膜结构和定位到A30 L开放阅读框的具有致密中心的病毒质病灶。一个单一的碱基变化，导致在残基17的非保守性丝氨酸-苯丙氨酸取代，与A30 L蛋白在高温下的降解。
Marker rescue experiments demonstrated that the genetic lesion of a previously isolated vaccinia virus temperature-sensitive mutant which forms multilayered envelope structures with lucent interiors and foci of viroplasm with dense centers mapped to the A30L open reading frame. A single base change, resulting in a nonconservative Ser-to-Phe substitution at residue 17, was associated with degradation of the A30L protein at elevated temperatures.