GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion
GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion
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DOI：
10.1016/j.bbrc.2016.01.168
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发表时间：
2016-02-26
影响因子：
3.1
通讯作者：
Liu, Bin
中科院分区：
文献类型：
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作者：
Chai, Peng;Tian, Jingzhong;Liu, Bin
Gse1 卷曲螺旋蛋白 (GSE1)，也称为 KIAA0182，是一种富含脯氨酸的蛋白质。然而，GSE1 的功能很大程度上未知。在这项研究中，我们报道了 GSE1 在乳腺癌中过度表达，GSE1 的沉默显着抑制了乳腺癌细胞的增殖、迁移和侵袭。此外，GSE1 被确定为 miR-489-5p 的直接靶标，miR-489-5p 在乳腺癌组织中显着减少。此外，miR-489-5p的强制表达可抑制乳腺癌细胞的增殖、迁移和侵袭。此外，siRNA 消除 GSE1 显着消除了 miR-489-5p 消除后乳腺癌细胞的增殖、迁移和侵袭增强。总而言之，这些发现表明 GSE1 可能作为乳腺癌中的一种新癌基因，并且可以受到 miR-489-5p 的调节。 (C) 2016 Elsevier Inc. 保留所有权利。
Gse1 coiled-coil protein (GSE1), also known as KIAA0182, is a proline rich protein. However, the function of GSE1 is largely unknown. In this study, we reported that GSE1 is overexpression in breast cancer and silencing of GSE1 significantly suppressed breast cancer cells proliferation, migration and invasion. Furthermore, GSE1 was identified as a direct target of miR-489-5p, which is significantly reduced in breast cancer tissues. In addition, forced expression of miR-489-5p suppressed breast cancer cells proliferation, migration and invasion. Moreover, depletion of GSE1 by siRNAs significantly abrogated the enhanced proliferation, migration and invasion of breast cancer cells consequent to miR-489-5p depletion. Taken together, these findings suggest that GSE1 may function as a novel oncogene in breast cancer and it can be regulated by miR-489-5p. (C) 2016 Elsevier Inc. All rights reserved.