The Effect of Cell Density on the Maturation and Contractile Ability of Muscle Derived Cells in a 3D Tissue-Engineered Skeletal Muscle Model and Determination of the Cellular and Mechanical Stimuli Required for the Synthesis of a Postural Phenotype
The Effect of Cell Density on the Maturation and Contractile Ability of Muscle Derived Cells in a 3D Tissue-Engineered Skeletal Muscle Model and Determination of the Cellular and Mechanical Stimuli Required for the Synthesis of a Postural Phenotype
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DOI：
10.1002/jcp.22271
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发表时间：
2010-12-01
影响因子：
5.6
通讯作者：
Lewis, M. P.
中科院分区：
文献类型：
--
作者：
Mudera, V.;Smith, A. S. T.;Lewis, M. P.
一个真正的骨骼肌仿生模型的成功工程可能对许多生物医学学科产生重大影响。尽管目前正在开发各种技术，但目前还没有广泛可用且易于复制的3D人工肌肉组织合成培养系统。在试图生成这样一个模型时，为了生成最能代表体内环境的组织，优化任何方案都是必不可少的。由于培养中肌肉来源细胞的成熟严重依赖于密度，因此在这些模型的开发中需要解决的一个主要因素是播种细胞以产生最佳反应的理想浓度。在研究细胞密度对已建立的基于3D胶原蛋白的骨骼肌模型中细胞性能的影响时，我们证明了在峰值力产生和肌源性基因表达数据方面确实存在最佳密度。然而，更大的密度导致形成更生理相关的组织，其表型特征为缓慢的姿势性肌肉。j .细胞。中国生物医学工程学报，2009,31(2):444 - 444。(C) 2010 Wiley-Liss, Inc。
The successful engineering of a truly biomimetic model of skeletal muscle could have a significant impact on a number of biomedical disciplines. Although a variety of techniques are currently being developed, there is, as of yet, no widely available and easily reproducible culture system for the synthesis of 3D artificial muscle tissues. In attempting to generate such a model it is essential to optimise any protocol in order to generate a tissue that best represents the in vivo environment. Since the maturation of muscle derived cells in culture is critically dependent on density, a major factor to be addressed in the development of these models is the ideal concentration at which to seed cells in order to generate an optimal response. In studying the effect of cell density on the performance of cells in an established 3D collagen based model of skeletal muscle, we demonstrate that an optimum density does exist in terms of peak force generation and myogenic gene expression data. Greater densities however, lead to the formation of a more physiologically relevant tissue with a phenotype characteristic of slow, postural muscle. J. Cell. Physiol. 225: 646-653, 2010. (C) 2010 Wiley-Liss, Inc.