Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes.
Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes.
复制标题
角度限制荧光光学投影断层扫描可定位淋巴结中的微转移。
DOI：
10.1117/1.jbo.24.11.110501
复制
发表时间：
2019
影响因子：
3.5
通讯作者：
Tichauer,KennethM
中科院分区：
文献类型：
--
作者：
Torres,VeronicaC;Li,Chengyue;He,Yusheng;Sinha,Lagnojita;Papavasiliou,Georgia;Sattar,HusainA;Brankov,JovanG;Tichauer,KennethM
淋巴结活检是乳腺癌分期的主要手段，但标准病理技术非常耗时，并且通常采样不到总淋巴结体积的 1%。演示了一种低成本荧光光学投影断层扫描 (OPT) 方案，可对整个淋巴结进行三维快速成像。通过采用光子检测的角度限制，可以利用淋巴结组织相对较低的散射特性来显着提高淋巴结 OPT 的空间分辨率。通过猪淋巴结转移模型证明，简单的滤波反投影重建足以检测和定位直径 1 厘米淋巴结中直径 200 微米的转移（具有临床意义的最小的转移）。
Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nodes in three dimensions. The relatively low scattering properties of lymph node tissue can be leveraged to significantly improve spatial resolution of lymph node OPT by employing angular restriction of photon detection. It is demonstrated through porcine lymph node metastases models that simple filtered-backprojection reconstruction is sufficient to detect and localize 200-μm-diameter metastases (the smallest clinically significant) in 1-cm-diameter lymph nodes.