Expression of heparanase in hepatocellular carcinoma has prognostic significance: A tissue microarray study
Expression of heparanase in hepatocellular carcinoma has prognostic significance: A tissue microarray study
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DOI：
10.3727/096504008785114138
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Tang, Xiao-Ling
中科院分区：
文献类型：
--
作者：
Chen, Gang;Dang, Yi-Wu;Tang, Xiao-Ling
乙酰肝素酶在肿瘤细胞的侵袭和转移中发挥着重要作用。在本研究中，我们探讨了乙酰肝素酶蛋白在肝细胞癌发生中的表达和临床病理意义，以探讨其在组织微阵列（TMA）中肝细胞癌（HCC）侵袭中的作用以及生物学行为与预后的关系。采用免疫组织化学法检测TMAs中乙酰肝素酶的表达情况，TMAs包括120例HCC、48例癌旁肝脏、62例肝硬化和23例正常肝组织。通过统计分析来了解乙酰肝素酶表达与 HCC 临床病理特征之间的相关性。结果显示，肝癌组织中乙酰肝素酶阳性水平显着高于癌旁肝、肝硬化及正常肝组织。乙酰肝素酶在临床 TNM I 和 II 期中的表达低于 III 和 IV 期。此外，20个月内未发生转移的病例中乙酰肝素酶的表达在统计学上低于发生转移的病例。此外，甲胎蛋白（AFP）>=400μg/L、门静脉瘤栓、多发肿瘤淋巴结、肿瘤直径≥5cm组的乙酰肝素酶表达显着高于相应组，而与患者的年龄、性别、组织学分类、肝硬化或肿瘤包膜浸润无关。 快速鉴定 HCC 的分子改变。乙酰肝素酶的过度表达可能在肝癌的发生、进展和转移中发挥重要作用。它可以作为临床预后和疗效的决定因素。
Heparanase plays an important role in invasion and metastasis of tumor cells. In this study, we explored the expression and clinicopathological significance of heparanase protein in hepatocellular carcinogenesis to investigate their roles in invasion and the relationship between biological behavior and prognosis of hepatocellular carcinoma (HCC) in tissue microarrays (TMAs). Heparanase expression was examined by immunohistochemistry in TMAs comprising 120 cases of HCC, 48 cases of adjacent tumor liver, 62 cases of cirrhosis, and 23 cases of normal liver tissues. Statistical analyses were determined to access the correlation between heparanase expression and the clinicopathological features of HCC. The results showed a positive level of heparanase in HCC tissues that was significantly higher than that in adjacent tumor liver, cirrhosis, and normal liver tissue. Heparanase was expressed lower in clinical TNM stages I and II than in III and IV. Moreover, the expression of heparanase in cases without metastasis within 20 months was statistically lower than in those with metastasis. Furthermore, heparanase expression in groups of (x-fetoprotein (AFP) >= 400 mu g/L, portal vein tumor emboli, multiple tumor nodes, and tumor diameter ! 5 cm were significantly higher than those of corresponding groups, while it was not associated with patients' age, sex, histological classification, cirrhosis, or tumor capsular infiltration. In conclusion, TMA is a powerful tool for the rapid identification of molecular alterations in HCC. The overexpression of heparanase may play an important role in hepatocarcinogenesis, progression, and metastases of HCC. It could serve as a determining factor for clinical prognosis and curative effect.