Large-Scale Fluorescence Calcium-Imaging Methods for Studies of Long-Term Memory in Behaving Mammals.
Large-Scale Fluorescence Calcium-Imaging Methods for Studies of Long-Term Memory in Behaving Mammals.
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DOI：
10.1101/cshperspect.a021824
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发表时间：
2016-05-02
影响因子：
7.2
通讯作者：
Schnitzer MJ
中科院分区：
文献类型：
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作者：
Jercog P;Rogerson T;Schnitzer MJ
在长期记忆形成过程中，细胞和分子过程重塑了单个神经元对突触输入的特定模式的反应。人们对这些变化如何影响哺乳动物神经元网络的信息处理仍然知之甚少。为了观察网络是如何编码、存储和检索信息的，神经科学家必须在与长期记忆相称的时间尺度上，跟踪行为动物中单个细胞的大集合的动态。荧光Ca2+成像技术可以监测行为小鼠的数百个神经元，为在网络水平上研究学习和记忆开辟了令人兴奋的途径。基因编码的Ca2+指示物允许神经元通过基因类型或连接成为目标。慢性动物制剂允许在数周内重复成像神经Ca2+动力学。总之，这些能力应该能够前所未有地分析整体神经编码如何在记忆处理过程中进化，并为记忆如何在大脑中组织提供新的见解。
During long-term memory formation, cellular and molecular processes reshape how individual neurons respond to specific patterns of synaptic input. It remains poorly understood how such changes impact information processing across networks of mammalian neurons. To observe how networks encode, store, and retrieve information, neuroscientists must track the dynamics of large ensembles of individual cells in behaving animals, over timescales commensurate with long-term memory. Fluorescence Ca2+-imaging techniques can monitor hundreds of neurons in behaving mice, opening exciting avenues for studies of learning and memory at the network level. Genetically encoded Ca2+ indicators allow neurons to be targeted by genetic type or connectivity. Chronic animal preparations permit repeated imaging of neural Ca2+ dynamics over multiple weeks. Together, these capabilities should enable unprecedented analyses of how ensemble neural codes evolve throughout memory processing and provide new insights into how memories are organized in the brain.