Early steps of V(D)J rearrangement: insights from biochemical studies of RAG-RSS complexes.
Early steps of V(D)J rearrangement: insights from biochemical studies of RAG-RSS complexes.
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DOI：
10.1007/978-1-4419-0296-2_1
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发表时间：
2009
影响因子：
--
通讯作者：
P. Swanson;Sushil Kumar;P. Raval
中科院分区：
文献类型：
--
作者：
P. Swanson;Sushil Kumar;P. Raval
V(D)J recombination is initiated by the synapsis and cleavage of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins. Our understanding of these processes has been greatly aided by the development of in vitro biochemical assays of RAG binding and cleavage activity. Accumulating evidence suggests that synaptic complex assembly occurs in a step-wise manner and that the RAG proteins catalyze RSS cleavage by mechanisms similar to those used by bacterial transposases. In this chapter we will review the molecular mechanisms of RAG synaptic complex assembly and 12/23-regulated RSS cleavage, focusing on recent advances that shed new light on these processes.
