Pulsed electromagnetic fields promote osteogenesis and osseointegration of porous titanium implants in bone defect repair through a Wnt/β-catenin signaling-associated mechanism.
Pulsed electromagnetic fields promote osteogenesis and osseointegration of porous titanium implants in bone defect repair through a Wnt/β-catenin signaling-associated mechanism.
复制标题
脉冲电磁场通过 Wnt/β-连环蛋白信号相关机制促进骨缺损修复中多孔钛植入物的成骨和骨整合。
DOI：
10.1038/srep32045
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发表时间：
2016-08-24
影响因子：
4.6
通讯作者：
Luo E
中科院分区：
文献类型：
--
作者：
Jing D;Zhai M;Tong S;Xu F;Cai J;Shen G;Wu Y;Li X;Xie K;Liu J;Xu Q;Luo E
骨缺损的治疗仍然是一个巨大的临床挑战。多孔钛合金（pTi）由于其良好的生物相容性和结构特性而成为理想的内种植体，但其骨整合不充分会导致种植体长期稳定性不稳定。大量证据表明，脉冲电磁场（PEMF）作为一种安全、无创的治疗方法，在实验和临床中均能抑制骨质减少/骨质疏松。我们在体外和体内研究了PEMF对pTi成骨和骨整合的效率和潜在机制。我们证明了PEMF增强了细胞的附着和增殖，并诱导了pTi中体外成骨细胞组织良好的细胞骨架。PEMF促进Runx2、OSX、COL-1和Wnt/β-catenin信号通路的基因表达。pemf刺激组Runx2、Wnt1、Lrp6和β-catenin蛋白表达升高。体内μCT和组织形态学分析结果显示，6周和12周的PEMF均能促进兔股骨骨缺损的成骨、成骨长入和pTi成骨率。PEMF促进Runx2、BMP2、OCN和Wnt/β-catenin信号通路的股骨基因表达。总之，我们证明了PEMF通过Wnt/β-catenin信号相关机制促进骨骼合成代谢活性，从而改善pTi的成骨和骨整合。PEMF有望成为提高骨缺损pTi修复效率和质量的一种有前景的生物物理方式。
Treatment of osseous defects remains a formidable clinical challenge. Porous titanium alloys (pTi) have been emerging as ideal endosseous implants due to the excellent biocompatibility and structural properties, whereas inadequate osseointegration poses risks for unreliable long-term implant stability. Substantial evidence indicates that pulsed electromagnetic fields (PEMF), as a safe noninvasive method, inhibit osteopenia/osteoporosis experimentally and clinically. We herein investigated the efficiency and potential mechanisms of PEMF on osteogenesis and osseointegration of pTi in vitro and in vivo. We demonstrate that PEMF enhanced cellular attachment and proliferation, and induced well-organized cytoskeleton for in vitro osteoblasts seeded in pTi. PEMF promoted gene expressions in Runx2, OSX, COL-1 and Wnt/β-catenin signaling. PEMF-stimulated group exhibited higher Runx2, Wnt1, Lrp6 and β-catenin protein expressions. In vivo results via μCT and histomorphometry show that 6-week and 12-week PEMF promoted osteogenesis, bone ingrowth and bone formation rate of pTi in rabbit femoral bone defect. PEMF promoted femoral gene expressions of Runx2, BMP2, OCN and Wnt/β-catenin signaling. Together, we demonstrate that PEMF improve osteogenesis and osseointegration of pTi by promoting skeletal anabolic activities through a Wnt/β-catenin signaling-associated mechanism. PEMF might become a promising biophysical modality for enhancing the repair efficiency and quality of pTi in bone defect.