The protist, Monosiga brevicollis, has a tyrosine kinase signaling network more elaborate and diverse than found in any known metazoan
The protist, Monosiga brevicollis, has a tyrosine kinase signaling network more elaborate and diverse than found in any known metazoan
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DOI：
10.1073/pnas.0801314105
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发表时间：
2008-07-15
影响因子：
11.1
通讯作者：
Zhai, Yufeng
中科院分区：
文献类型：
--
作者：
Manning, Gerard;Young, Susan L.;Zhai, Yufeng
酪氨酸激酶信号一直被认为是多细胞动物特有的细胞间通讯的标志。我们的单细胞choanoflagellate Monosiga brevicollis的基因组分析发现一个显着的计数128酪氨酸激酶，38酪氨酸磷酸酶，和123磷酸酪氨酸（pTyr）结合SH2蛋白，所有更高的计数比任何后生动物。这个精心制作的信号网络显示出与后生动物对应物几乎没有同源性，但显示出许多让人想起后生动物的创新。这些包括结构上与后生动物受体激酶相关的细胞外结构域，细胞质激酶中膜锚定和磷酸酪氨酸相互作用的替代方法，以及将激酶与小GT3信号传导和转录连接的结构域组合。这些蛋白质还显示出丰富的已知信号传导结构域的组合。这种独特的发散和精心设计的信号网络阐明了pTyr信号的早期演变，探索了穿越细胞信号通路的创新方法，并显示了广泛的收敛进化，突出了pTyr信号的普遍约束。
Tyrosine kinase signaling has long been considered a hallmark of intercellular communication, unique to multicellular animals. Our genomic analysis of the unicellular choanoflagellate Monosiga brevicollis discovers a remarkable count of 128 tyrosine kinases, 38 tyrosine phosphatases, and 123 phosphotyrosine (pTyr)-binding SH2 proteins, all higher counts than seen in any metazoan. This elaborate signaling network shows little orthology to metazoan counterparts yet displays many innovations reminiscent of metazoans. These include extracellular domains structurally related to those of metazoan receptor kinases, alternative methods for membrane anchoring and phosphotyrosine interaction in cytoplasmic kinases, and domain combinations that link kinases to small GTPase signaling and transcription. These proteins also display a wealth of combinations of known signaling domains. This uniquely divergent and elaborate signaling network illuminates the early evolution of pTyr signaling, explores innovative ways to traverse the cellular signaling circuitry, and shows extensive convergent evolution, highlighting pervasive constraints on pTyr signaling.