Physiological root responses of iron deficiency susceptible and tolerant tomato genotypes and their reciprocal F1 hybrids
Physiological root responses of iron deficiency susceptible and tolerant tomato genotypes and their reciprocal F1 hybrids
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DOI：
10.1023/a:1016060710288
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发表时间：
2002-04-01
期刊：
影响因子：
4.9
通讯作者：
Abak, K
中科院分区：
文献类型：
--
作者：
Dasgan, HY;Römheld, V;Abak, K
利用番茄基因型227/1（铁萎黄易感）和Roza（铁萎黄耐受性）及其互反F(1)杂种，在3种不同铁供应的营养液中，在受控的环境条件下，研究了根系形态变化、营养液pH变化、Fe- iii还原能力以及Fe-59的吸收和根尖转运情况：铁EDDHA(即10(-7)M，严重缺铁；10(-6) M，中间缺铁；10(-4) M，充足的铁供应)。从叶片萎黄的严重程度来看，耐铁亲本“罗扎”受低铁供应的影响小于敏感亲本“227/1”。在两种缺铁条件下，互交杂交种的黄化现象没有差异。在中度缺铁条件下，互易F-1杂交亲本（‘227/1 × Roza’和‘Roza × 227/1’）在耐感亲本和敏感亲本之间表现出中度黄化。然而，在严重缺铁的情况下，无论哪个亲本是细胞质的供给者，互易杂交种的褪绿性都比耐受亲本强。预培养期间铁供应的减少增强了亲本和互交杂交种的(FeII)-I-I还原能力。对铁缺乏的耐受性差异与基因型的(FeII)-I-I还原能力的相关性大于与铁缺乏诱导的H+离子释放的相关性。在两种缺铁条件下，耐铁亲本Roza对FeII - i的还原能力均明显高于敏感亲本227/1。在缺铁条件下，杂种‘Roza x’系227/1的还原能力与亲本Roza非常接近，但高于‘Roza x’系227/1的还原能力。在两种缺铁条件下，耐铁亲本的侧根数量均高于感铁亲本。在互易杂交种中，在缺铁条件下，Roza x系227/1比Roza x系227/1拥有更多的侧根。低铁营养状态导致根系对(5)9Fe的吸收显著增加。在铁供应充足的情况下，互易杂交种和亲本对铁的吸收和根到茎的转运没有差异。但在缺铁条件下，耐铁黄化品种对(5)9Fe的吸收和根尖转运显著高于敏感亲本。根据(FeII)-I-I的还原能力以及Fe的吸收和根-梢易位分析，母本基因型为Roza的F-1杂种比母本基因型为227/1的F-1杂种的耐受性更强。‘Roza x 227/1系’获得的F-1杂交种的吸收和易位率与亲本Roza相似，但高于‘227/1 × Roza’获得的F-1杂交种，特别是在中度缺铁条件下。结果表明，(FeII)-I-I还原比缺铁诱导的H+离子释放与铁效率的关系更好。罗扎耐缺铁性的遗传似乎不是简单的单基因遗传。它可能具有核遗传和核外遗传两种特征。‘227/1 × Roza’互易杂交种的中间反应表明，Roza耐缺铁性状可通过核遗传转移。‘Roza × 227/1’杂交种的抗旱性优于‘227/1 × Ro’杂交种
By using two tomato genotypes line 227/1 (Fe chlorosis susceptible) and Roza (Fe chlorosis tolerant) and their reciprocal F(1)hybrid, some root morphological changes, pH changes of nutrient solution, reduction capacity of Fe-III and uptake and root-to-shoot translocation of Fe-59 were studied under controlled environmental conditions in nutrient solution with 3 different Fe supplies as Fe EDDHA (i.e., 10(-7) M, severe Fe deficiency; 10(-6) M, intermediate Fe deficiency; 10(-4) M, adequate Fe supply). Tolerant parent `Roza' was less affected by low Fe supply than susceptible parent `line 227/1' as judged from the severity of leaf chlorosis. Under both Fe deficient conditions there were no differences between the reciprocal hybrids concerning the appearance of chlorosis. Under intermediate Fe deficiency, reciprocal F-1 hybrids (`line 227/1 x Roza' and `Roza x line' 227/1) showed an intermediate chlorosis between tolerant and susceptible parents. However, under severe Fe deficiency the reciprocal hybrids were more chlorotic than the tolerant parent irrespective of which parent was the cytoplasm contributor. A decreased Fe supply during preculture enhanced (FeII)-I-I reduction capacities of the parents and reciprocal hybrids. Differences in the tolerance to Fe deficiency always were better correlated with (FeII)-I-I reduction capacity of the genotypes than the Fe deficiency-induced release of H+ ions. Under both Fe deficient conditions the tolerant parent Roza had a much higher (FeII)-I-I reduction capacity than the susceptible parent line 227/1. The reduction capacity of the hybrids `Roza x line 227/1' was very similar to the capacity of the parent Roza, but higher than the capacity of the hybrids `line 227/1xRoza' at both Fe-deficient conditions. Under both Fe deficient conditions tolerant parent had higher number of lateral roots than the susceptible parent. Among the reciprocal hybrids `Roza x line 227/1' possessed more lateral roots than the `line 227/1 x Roza' under both Fe deficient conditions. Low Fe nutritional status resulted in marked increase in root uptake of (5)9Fe. At adequate Fe supply, reciprocal hybrids and their parents did not differ in uptake and root-to-shoot translocation of Fe. However, under Fe-deficient conditions uptake and root-to-shoot translocation of (5)9Fe were significantly higher in the Fe chlorosis tolerant than the susceptible parent. Based on the reduction capacity of (FeII)-I-I and uptake and root-to-shoot translocation of Fe, the F-1 hybrids obtained from the cross in which the maternal genotype was Roza appeared to be more tolerant than when the maternal genotype was the susceptible line 227/1. Uptake and translocation ratio of the F-1 hybrids obtained from `Roza x line 227/1' were similar to those of the parent Roza, but higher than the F-1 hybrids obtained from `line 227/1 x Roza', particularly under intermediate Fe deficiency. The results indicate that (FeII)-I-I reduction show a better relationship to Fe efficiency than Fe deficiency induced release of H+ ions. The inheritance of Fe deficiency tolerance of Roza seems not to be simple monogenic. It might be characterised by both, nuclear and extranuclear heredity. The intermediate responses of the reciprocal hybrids of the `line 227/1 x Roza' indicates that the Fe deficiency tolerance character of Roza is transferable by nuclear heredity. The better responses of the hybrids of `Roza x line 227/1' than the hybrids of `line 227/1 x Ro