Protein-interaction modules that organize nuclear function:: FF domains of CA150 bind the phosphoCTD of RNA polymerase II
Protein-interaction modules that organize nuclear function:: FF domains of CA150 bind the phosphoCTD of RNA polymerase II
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DOI：
10.1073/pnas.160266597
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发表时间：
2000-08-01
影响因子：
11.1
通讯作者：
Greenleaf, AL
中科院分区：
文献类型：
--
作者：
Carty, SM;Goldstrohm, AC;Greenleaf, AL
开发了一种用于纯化直接与RNA聚合酶II的高度磷酸化C-末端重复结构域（CTD）结合的核蛋白的方法，并用于鉴定一种人磷酸CTD相关蛋白CA 150。CA 150是一种参与转录延伸的核因子，因为过度磷酸化的CTD是活跃转录RNA聚合酶II（Pol II）的特征，所以磷酸CTD（PCTD）结合将CA 150置于适合于在转录延伸相关事件中发挥作用的位置。CA 150的几个重组片段与PCTD结合，主要结合由含有6个FF结构域的CA 150部分介导，FF结构域是以前未知功能的紧凑模块。事实上，含有第五个Pr结构域的小重组蛋白结合PCTD，PCTD结合是分配给Pr结构域的第一个特异性功能。由于FF结构域存在于多种核蛋白中，因此这些蛋白中的一些很可能也是PCD相关蛋白。因此FF结构域似乎是紧凑的蛋白质相互作用模块，像WW结构域一样，可以进化地改组以组织核功能。
An approach for purifying nuclear proteins that bind directly to the hyperphosphorylated C-terminal repeat domain (CTD) of RNA polymerase II was developed and used to identify one human phosphoCTD-associating protein as CA150. CA150 is a nuclear factor implicated in transcription elongation, Because the hyperphosphorylated CTD is a feature of actively transcribing RNA polymerase II (Pol II), phosphoCTD (PCTD) binding places CA150 in a location appropriate for performing a role in transcription elongation-related events. Several recombinant segments of CA150 bound the PCTD, Predominant binding is mediated by the portion of CA150 containing six FF domains, compact modules of previously unknown function. In fact, small recombinant proteins containing the fifth Pr domain bound the PCTD, PCTD binding is the first specific function assigned to an Pr domain. As FF domains are found in a variety of nuclear proteins, it is likely that some of these proteins are also PCTD-associating proteins. Thus FF domains appear to be compact protein-interaction modules that, like WW domains, can be evolutionarily shuffled to organize nuclear function.