A workflow for multiclass determination of 256 pesticides in essential oils by liquid chromatography tandem mass spectrometry using evaporation and dilution approaches: Application to lavandin, lemon and cypress essential oils.
A workflow for multiclass determination of 256 pesticides in essential oils by liquid chromatography tandem mass spectrometry using evaporation and dilution approaches: Application to lavandin, lemon and cypress essential oils.
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使用蒸发和稀释方法，通过液相色谱串联质谱法对精油中 256 种农药进行多类测定的工作流程：应用于薰衣草、柠檬和丝柏精油。
DOI：
10.1016/j.talanta.2015.11.052
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
P. Communal
中科院分区：
文献类型：
--
作者：
Yoann Fillâtre;D. Rondeau;Antoine Daguin;P. Communal
本文描述了通过液相色谱-电喷雾电离串联质谱 (LC-ESI/MS/MS) 分析方法，使用混合四极杆线性离子阱 (QLIT) 质谱仪上的预定选择反应监测模式 (sSRM) 来测定柏树和柠檬精油 (EO) 中的 256 种多类农药。评估了基于受控加热辅助的氮气下稀释或蒸发的柠檬和柏树 EO 样品制备的性能。通过氮气蒸发法实现了最佳定量限 (LOQ)，91% 的农药的 LOQs≤10 µg L−1。此外，在回收率、重复性和线性方面获得的非常令人满意的结果表明，对于蒸发温度相对较低的环氧乙烷，基于氮气下蒸发的样品制备非常适合并且优于稀释。通过将这些结果与我们中的一些人之前发表的薰衣草精油 EO 的结果进行汇编，我们提出了一个专门用于通过 LC-ESI/sSRM 测定各种 EO 中农药多残留的工作流程。在该工作流程涉及的步骤中，与质谱相关的协议提出了一种经典 SRM 比率标准的替代确认方法，该方法基于 sSRM 调查扫描，然后使用敏感增强子离子 (EPI) 扫描进行信息相关采集，以生成 MS/MS 谱图，然后与参考进行比较。提交的工作流程适用于柠檬环氧乙烷样品，首次强调了在一个环氧乙烷中同时检测 20 种多类农药。一些农药的浓度非常高，远远超过 mg L−1。
This paper describes the determination of 256 multiclass pesticides in cypress and lemon essential oils (EOs) by the way of liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI/MS/MS) analysis using the scheduled selected reaction monitoring mode (sSRM) available on a hybrid quadrupole linear ion trap (QLIT) mass spectrometer. The performance of a sample preparation of lemon and cypress EOs based on dilution or evaporation under nitrogen assisted by a controlled heating were assessed. The best limits of quantification (LOQs) were achieved with the evaporation under nitrogen method giving LOQs≤10 µg L−1for 91% of the pesticides. In addition the very satisfactory results obtained for recovery, repeatability and linearity showed that for EOs of relatively low evaporation temperature, a sample preparation based on evaporation under nitrogen is well adapted and preferable to dilution. By compiling these results with those previously published by some of us on lavandin EO, we proposed a workflow dedicated to multiresidue determination of pesticides in various EOs by LC–ESI/sSRM. Among the steps involved in this workflow, the protocol related to mass spectrometry proposes an alternative confirmation method to the classical SRM ratio criteria based on a sSRM survey scan followed by an information-dependent acquisition using the sensitive enhanced product ion (EPI) scan to generate MS/MS spectra then compared to a reference. The submitted workflow was applied to the case of lemon EOs samples highlighting for the first time the simultaneous detection of 20 multiclass pesticides in one EO. Some pesticides showed very high concentration levels with amounts greatly exceeding the mg L−1.