[Therapeutic potential of n-butanol extract of Pulsatilla decoction in a murine model of ulcerative colitis induced by DSS combined with Candida albicans colonization].
[Therapeutic potential of n-butanol extract of Pulsatilla decoction in a murine model of ulcerative colitis induced by DSS combined with Candida albicans colonization].
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白头翁水煎正丁醇提取物对DSS联合白色念珠菌定植诱导的溃疡性结肠炎小鼠模型的治疗潜力
DOI：
10.19540/j.cnki.cjcmm.20180417.001
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chang
中科院分区：
文献类型：
--
作者：
Xiao;Ge Tian;Qiang;Daqiang Wu;J. Shao;Tian;Chang
为探讨白芍汤正丁醇提取物（BAEB）抗DSS联合白色念珠菌（CA）定植所致小鼠溃疡性结肠炎（UC）模型的作用机制，将小鼠随机分为正常对照组、DSS组、DSS+CA组、BAEB高、中、低剂量组和阳性药物美沙嗪组。观察小鼠一般情况，平板法检测小鼠肠道内容物真菌负荷，HE染色观察小鼠结肠病理改变。ELISA法检测血清中ASCA及结肠黏膜中IL-6、IL-8、IL-1β、HBD-2、HBD-3。结果表明，与DSS组相比，DSS+CA组大鼠的一般情况和血清ASCA无明显变化，但肠道内容物真菌负荷、结肠病理损伤及结肠黏膜IL-6、IL-8、IL-1β、HBD-2、HBD-3水平均高于DSS组。大剂量BAEB组和美沙拉嗪组可改善结肠病理，降低IL-6、IL-8、IL-1β、HBD-2、HBD-3表达水平。综上所述，BAEB可有效改善DSS联合CA定植诱导的小鼠UC症状，并抑制IL-6等炎症因子，提示BAEB在治疗肠道真菌相关性结肠炎中具有重要价值。
To investigate the mechanism of n-butanol extract of Pulsatilla decoction (BAEB) against murine ulcerative colitis (UC) model induced by DSS combined with Candida albicans (CA) colonization, mice were randomly divided into normal control group, DSS group, DSS+CA group, BAEB high, medium and low dose group, and positive drug Mesalazine group. The general condition of mice was observed, fungal loads of murine intestinal contents were detected by plate method, colonic pathological change of mice was examined by HE staining. ASCA in serum and IL-6, IL-8, IL-1β, HBD-2, HBD-3 in colonic mucosa were detected by ELISA. The results showed that, compared with DSS group, the general condition and ASCA in serum had no obvious change for DSS+CA group, but the fungal loads in intestinal contents, the colonic pathological damage, and the levels of IL-6, IL-8, IL-1β, HBD-2, HBD-3 in colonic mucosa were greater than that of DSS group. High dose of BAEB group and Mesalazine group could improve the colonic pathology, decrease IL-6, IL-8, IL-1β, HBD-2, HBD-3 expression level. In conclusion, BAEB could effectively improve the UC symptoms in mice induced by DSS combined with CA colonization, and inhibit the inflammatory factors such as IL-6, imply that BAEB is of important value for the treatment of intestinal fungal-related colitis.