Flow cytometric analysis of lymphoma and lymphoma-like disorders.
Flow cytometric analysis of lymphoma and lymphoma-like disorders.
复制标题
淋巴瘤和淋巴瘤样疾病的流式细胞术分析。
DOI：
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发表时间：
1989
影响因子：
2.3
通讯作者：
S. Crago
中科院分区：
文献类型：
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作者：
J. Little;K. Foucar;A. Horváth;S. Crago
流式细胞术(FC)的应用代表了淋巴细胞亚群表型分析的最新进展，并已成为诊断恶性非霍奇金淋巴瘤(NHL)的有价值的辅助手段。在对新墨西哥大学免疫表型实验室研究的200多例结节和结外疑似淋巴瘤病例的回顾中，对FC的诊断效用进行了评估。在NHL病例中，FC能够确认淋巴瘤的形态诊断，并在提交的样本中确定超过85%的B或T细胞谱系。其余的形态非霍奇金淋巴瘤患者的家系确定困难是多因素的。在反应性淋巴结和霍奇金氏病的病例中，FC没有表现出特征性的模式，尽管一些病例表现出B或T细胞克隆性的表型特征。当结合常规的形态学检查并在必要时辅以其他专门的诊断技术时，FC的使用代表了一种快速、容易地研究实体组织中淋巴增生性疾病的精确和可重复性的方法。
The use of flow cytometry (FC) represents the most recent advance in the phenotypic analysis of lymphocyte subsets, and has emerged as a valuable adjunct in the diagnosis of malignant non-Hodgkin's lymphoma (NHL). In a review of over 200 cases of nodal and extranodal suspected lymphomas studied in the Immunophenotyping Laboratory at the University of New Mexico, the diagnostic utility of FC was assessed. Among cases of NHL, FC was able to confirm a morphologic diagnosis of lymphoma and determine B or T cell lineage in greater than 85% of the samples submitted. Difficulty in lineage determination in the remaining cases of morphologic NHL was multifactorial. Among cases of reactive lymph nodes and Hodgkin's disease, FC showed no characteristic patterns, although several cases exhibited phenotypic profiles suggestive of B or T cell clonality. When combined with routine morphologic review and accompanied by other specialized diagnostic techniques when necessary, the use of FC represents a precise and reproducible method for rapidly and easily studying lymphoproliferative disorders in solid tissue.