Functional Localization of an Attenuating Filter within Cortex for a Selective Detection Task in Mice
Functional Localization of an Attenuating Filter within Cortex for a Selective Detection Task in Mice
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DOI：
10.1523/jneurosci.2993-19.2020
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发表时间：
2020-07-08
影响因子：
5.3
通讯作者：
Zagha, Edward
中科院分区：
文献类型：
--
作者：
Aruljothi, Krithiga;Marrero, Krista;Zagha, Edward
目标导向行为的一个基本特征是有选择地对环境中的各种刺激作出反应的能力。然而，使我们能够对目标刺激作出反应而忽略干扰刺激的神经机制尚不清楚。为了研究这种感觉选择过程，我们在一个选择性检测任务中训练雄性和雌性小鼠，在这个任务中，小鼠学会对目标须域中的快速刺激做出反应，而忽略相反的干扰须域中的相同刺激。在专家小鼠中，我们使用宽视场Ca2+成像来分析整个背皮质的靶相关和分心相关神经反应。对于目标刺激，我们观察到初级体感皮层（S1）和额叶皮层（包括初级运动皮层（wMC）和前外侧运动皮层（ALM）的须状区域）的强信号激活。对于分心物刺激，我们观察到S1的强信号激活，但向额叶皮层的传播很少。我们的数据仅支持适度的皮层下过滤，在Si和wMC的单突触耦合区域之间的分心物处理中具有鲁棒的阶梯状衰减。本研究为研究感觉选择的神经机制建立了一个高度稳健的模型系统，并对其实施提出了重要的约束条件。
An essential feature of goal-directed behavior is the ability to selectively respond to the diverse stimuli in one's environment. However, the neural mechanisms that enable us to respond to target stimuli while ignoring distractor stimuli are poorly understood. To study this sensory selection process, we trained male and female mice in a selective detection task in which mice learn to respond to rapid stimuli in the target whisker field and ignore identical stimuli in the opposite, distractor whisker field. In expert mice, we used widefield Ca2+ imaging to analyze target-related and distractor-related neural responses throughout dorsal cortex. For target stimuli, we observed strong signal activation in primary somatosensory cortex (S1) and frontal cortices, including both the whisker region of primary motor cortex (wMC) and anterior lateral motor cortex (ALM). For distractor stimuli, we observed strong signal activation in S1, with minimal propagation to frontal cortex. Our data support only modest subcortical filtering, with robust, step-like attenuation in distractor processing between mono-synaptically coupled regions of Si and wMC. This study establishes a highly robust model system for studying the neural mechanisms of sensory selection and places important constraints on its implementation.