Molecular association of trinitrobenzenesulfonic acid and surface phospholipids in the development of colitis in rats.

Molecular association of trinitrobenzenesulfonic acid and surface phospholipids in the development of colitis in rats.
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三硝基苯磺酸和表面磷脂在大鼠结肠炎发展中的分子关联。

DOI：
10.1053/gast.1996.v110.pm8608888
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发表时间：
1996
期刊：
Gastroenterology
影响因子：
29.4
通讯作者：
L. Lichtenberger
L. Lichtenberger
中科院分区：
医学1区
文献类型：
--
作者：
Yoshihide Tatsumi;L. Lichtenberger

文献摘要

被引文献

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背景与目标 粘膜疏水性有助于胃的屏障性能。本研究探讨了 2,4,6-三硝基苯磺酸 (TNBS) 对结肠粘膜疏水性的影响及其与炎症发展的关系。 方法 初步研究表明 TNBS 可以与两性离子磷脂发生化学反应，在将大鼠结肠外翻囊管腔暴露于 TNBS 后，对结肠粘膜进行接触角分析。在体内，通过灌肠给予大鼠TNBS、乙醇中的TNBS或TNBS与二棕榈酰磷脂酰胆碱偶联。第11天，测量粘膜疏水性损伤评分、结肠重量和髓过氧化物酶活性。 结果 当在 50% 乙醇中施用时，TNBS 在体外和体内均急剧（5 分钟）降低大鼠结肠粘膜的接触角。结肠内接种 TNBS 乙醇溶液 11 天后，表面粘膜疏水性显着降低，并且似乎与结肠炎症有关。如果半抗原与二棕榈酰磷脂酰胆碱偶联，TNBS 降低粘膜疏水性和诱导结肠炎的能力就会减弱。 结论 TNBS 可通过与结肠粘膜的表面活性磷脂反应来降低粘膜疏水性。结肠粘膜疏水完整性降低可能导致 TNBS 诱导的结肠炎症。
BACKGROUND & AIMS Mucosal hydrophobicity contributes to the barrier property of the stomach. This study examined the effect of 2,4,6-trinitrobenzenesulfonic acid (TNBS) on colonic mucosal hydrophobicity and its relationship with developing inflammation. METHODS After the initial study, which suggested that TNBS can chemically react with zwitterionic phospholipids, contact-angle analysis of the colonic mucosa was performed after the luminal exposure of everted sacs of rat colon to TNBS. In vivo, rats were given TNBS, TNBS in ethanol, or TNBS coupled with dipalmitoylphosphatidylcholine by enema. On the 11th day, mucosal hydrophobicity damage score, colon weight, and myeloperoxidase activity were measured. RESULTS TNBS acutely (5 minutes) reduced the contact angle of the rat colonic mucosa in vitro as well as in vivo when administered in 50% ethanol. Surface mucosal hydrophobicity was significantly reduced 11 days after intracolonic inoculation with TNBS in ethanol and appeared to be associated with colonic inflammation. The ability of TNBS to decrease mucosal hydrophobicity and induce colitis was attenuated if the hapten was coupled with dipalmitoylphosphatidylcholine. CONCLUSIONS TNBS may reduce mucosal hydrophobicity by reacting with the surface-active phospholipids of the colonic mucosa. Reduced hydrophobic integrity of the colonic mucosa may contribute to TNBS-induced colonic inflammation.