Effect of mild hypothermia on angiogenesis in rats with focal cerebral ischemia
Effect of mild hypothermia on angiogenesis in rats with focal cerebral ischemia
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DOI：
10.1016/j.neulet.2007.03.072
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发表时间：
2007-07-11
影响因子：
2.5
通讯作者：
Ye, Fei
中科院分区：
文献类型：
--
作者：
Xie, Yan-Chun;Li, Cheng-Yan;Ye, Fei
在本研究中，我们研究了亚低温对脑卒中后梗死体积、血管生成和脑源性神经营养因子（BDNF）水平的影响。采用氯化三苯基四氮唑（TTC）染色、激光扫描共聚焦显微镜（LSCM）和酶联免疫吸附试验（ELISA）分别检测脑梗死体积、缺血边界区（IBZ）血管生成和BDNF水平。与常温组相比，亚低温组脑梗死体积减小，内源性BDNF水平升高。亚低温组微血管直径、血管分支点数、血管表面积均明显增加。这些结果表明，亚低温促进缺血脑血管新生，这可能是通过BDNF部分增强。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
In the present study, we investigated the effect of mild hypothermia on infarct volume, angiogenesis and brain-derived neurotrophic factor (BDNF) level after stroke. After permanent middle cerebral artery occlusion, mild hypothermia was induced immediately and maintained for 24 It. 2,3,5-Triphenyltetrazolium chloride (TTC) staining, laser scanning confocal microscopy (LSCM) and ELISA were performed to assay infarct volume, angiogenesis and BDNF level in the ischemic boundary zone (IBZ), respectively. Compared with normothermic group, mild hypothermia reduced total infarct volume and increased endogenous BDNF level. And the microvessel diameter, the number of vascular branch points and the vessel surface area were significantly increased in the mild hypothermia group. These findings suggest that mild hypothermia enhances angiogenesis in ischemic brain, which might be enhanced in part via BDNF. (c) 2007 Elsevier Ireland Ltd. All rights reserved.