REGULATION OF POLYPHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C ACTIVITY BY PURIFIED GQ
REGULATION OF POLYPHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C ACTIVITY BY PURIFIED GQ
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DOI：
10.1126/science.1846707
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发表时间：
1991-02-15
期刊：
影响因子：
56.9
通讯作者：
STERNWEIS, PC
中科院分区：
文献类型：
--
作者：
SMRCKA, AV;HEPLER, JR;STERNWEIS, PC
磷脂酶 C 水解磷脂酰肌醇 4,5-二磷酸 (PIP2) 产生第二信使肌醇 1,4,5-三磷酸 (InsP3) 和 1,2-二酰基甘油。 这种活性通过 G 蛋白途径受到多种激素的调节。 然而，具体的 G 蛋白或所涉及的蛋白质尚未确定。最近，新发现的百日咳毒素不敏感 G 蛋白 (G(q)) 的 α 亚基已被分离出来，现已证明可以刺激牛脑中多磷酸肌醇特异性磷脂酶 C (PI-PLC) 的活性。 PI-PLC的最大活性和对钙离子的亲和力均受到影响。 这些结果将 G(q) 确定为调节 PI-PLC 的 G 蛋白。
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and 1,2-diacylglycerol. This activity is regulated by a variety of hormones through G protein pathways. However, the specific G protein or proteins involved has not been identified. The alpha-subunit of a newly discovered pertussis toxin-insensitive G protein (G(q)) has recently been isolated and is now shown to stimulate the activity of polyphosphoinositide-specific phospholipase C (PI-PLC) from bovine brain. Both the maximal activity and the affinity of PI-PLC for calcium ion were affected. These results identify G(q) as a G protein that regulates PI-PLC.