CROSS-TALK AMONG ROR-ALPHA-1 AND THE REV-ERB FAMILY OF ORPHAN NUCLEAR RECEPTORS
CROSS-TALK AMONG ROR-ALPHA-1 AND THE REV-ERB FAMILY OF ORPHAN NUCLEAR RECEPTORS
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DOI：
10.1210/me.8.9.1253
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发表时间：
1994-09-01
影响因子：
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通讯作者：
EVANS, RM
中科院分区：
文献类型：
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作者：
FORMAN, BM;CHEN, J;EVANS, RM
我们已经克隆了Rev-erb β，一种新的Rev-erb α孤儿核受体亚型。Rev-erb α和β的DNA结合结构域彼此高度相关，并且与视黄酸相关孤儿受体（ROR）/核受体RZR亚家族高度相关。事实上，我们发现所有三种受体都作为单体与序列AATGT-AGGTCA结合。尽管ROR α 1通过该序列组成性地激活转录，但Rev-erb的两种同种型都是无活性的。当共表达时，两种Rev-erb亚型均抑制ROR α 1的转录活性。我们的数据将Rev-erb和ROR/RZR定义为在重叠的调控网络上具有相反活性的相关受体家族。
We have cloned Rev-erb beta, a novel isoform of the Rev-erb alpha orphan nuclear receptor. The DNA binding domains of Rev-erb alpha and beta are highly related to each other and to the retinoic acid related orphan receptor (ROR)/RZR subfamily of nuclear receptors. Indeed, we find that all three receptors bind as monomers to the sequence AATGT-AGGTCA. Whereas ROR alpha 1 constitutively activates transcription through this sequence, both isoforms of Rev-erb are inactive. When coexpressed, both Rev-erb isoforms suppress the transcriptional activity of ROR alpha 1. Our data define Rev-erb and ROR/RZR as a family of related receptors with opposing activities on overlapping regulatory networks.